CURVATURE ELASTICITY IN OUTER HAIR CELL ELECTROMOTILITY

外毛细胞电动性的曲率弹性

• 批准号: 6094168
• 负责人: ROBERT M RAPHAEL
• 金额: $ 3.84万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6094168
• 关键词: biomechanics; cell motility; cochlear microphonic potentials; dielectric property; ear hair cell; elasticity; membrane potentials; voltage /patch clamp

The objective of the research in this proposal is to understand the molecular mechanism of outer hair cell electromotility. This unique form of cellular motility is responsible for the remarkable sensitivity and exquisite frequency selectivity of mammalian hearing. Loss of electromotility is believed to be responsible for sensioneural hearing loss. Until we understand the molecular mechanism of electromotility, we will be powerless to undertake any clinical efforts to treat people suffering from hearing impairment. The research in this proposal plans to test the novel hypothesis that changes in membrane curvature are responsible for the electromechanical coupling process. This will be accomplished experimentally by testing the effects of various agents known to affect the ability of a membrane to bend on the outer hair cell's voltage dependent capacitance and motility. A theoretical model of electromotility will be developed which includes an analytic expression for the curvature elastic energy. This model will be used to predict the shift in the nonlinear capacitance with membrane tension. The model will also be used to predict the capacitance change expected from membrane bending which will be calculated from the Poisson- Boltzmann equation and membrane electrostatic theory.

该提案中研究的目的是了解 外毛细胞电动性的分子机制。 这个独特 细胞运动的形式是显着灵敏度的原因 和哺乳动物听力的精致频率选择性。 损失 据信电动性负责轰动性听力 损失。 直到我们了解电动性的分子机制 我们将无能为力地进行对待人的任何临床努力 遭受听力障碍。 该提案计划中的研究 测试新的假设，即膜曲率变化是 负责机电耦合过程。 这将是 通过测试各种试剂的效果实验完成 已知会影响膜在外发上弯曲的能力 电池的电压依赖性电容和运动能力。 理论模型 将开发电动性，其中包括一个分析 曲率弹性能的表达。 该模型将被使用 通过膜张力预测非线性电容的变化。 该模型还将用于预测预期的电容变化 从膜弯曲中，将从泊松中计算出来 Boltzmann方程和膜静电理论。