MOLECULAR ASPECTS OF MRI CONTRAST AGENTS SELECTIVITY

对比增强磁共振成像（MRI）是一种非常有效的 临床诊断技术 然而，大多数顺磁性造影剂 (PCA)纯粹基于非特异性生物学增强图像对比度， 分布特性，这有时足以可视化一些 生化事件 目前，在理解上存在一些差距， PCA结构-功能关系，可逆 五氯苯甲醚与生物小分子的结合，以及 环境对PCA弛豫率的影响。 建议的广泛目标 项目是了解分子方面的结合和相互作用的 为临床MRI应用开发的PCA分子具有几个关键 生物分子，如血浆蛋白和磷脂，以及 结合和弛豫增强之间的关系。 我们的目标是 通过表征结合相互作用和研究 这些相互作用对结构和电子性质的影响 通过对几类Gd ~（3+）配合物的系统研究， 各种光谱技术，包括荧光，核 磁共振和电子顺磁共振在多个和 高频率 这一关键信息对于指导开发至关重要 新的、更有效的造影剂，具有增强的弛豫性， 选择性

Contrast-enhanced magnetic resonance imaging (MRI) is a very effective clinical diagnostic technique. However, most paramagnetic contrast agents (PCA) enhance image contrast purely on the basis of nonspecific bio- distribution properties, which is sometimes enough to visualize some biochemical events. Currently, there are some gaps in understanding of the structure-function relationship of PCA, molecular mechanism of reversible binding of PCA to biological micromolecules, and the effect of biological environment on relaxivity of PCA. The broad objectives of the proposed project are to understand molecular aspects of binding and interactions of PCA molecules developed for clinical MRI applications with several key biomolecules, such as plasma proteins and phospholipids, and the relationship between binding and relaxation enhancement. The goal will be achieved by characterization of binding interaction and by investigating effects of these interactions on structure and electronic properties of PCA's through systematic study of several classes Gd3+ complexes by a variety of spectroscopic techniques, including fluorescence, nuclear magnetic resonance, and electron paramagnetic resonance at multiple and high frequencies. This key information is essential to guide development of new, more effective contrast agents with enhanced relaxivity and selectivity.