INTERACTION OF MRI GD CONTRAST AGENTS W/ MEMBRANES AS STUDIED BY HF EPR

两种磁共振成像Gd造影剂的相互作用 乙氧基苄基二乙三胺五乙酸酯(Gd-EOB-DTPA)和 2-戊基-(1，4，7，10-四氮杂环十二烷-N，N‘，N’‘， N‘-四乙酸)(Gd-DOTA-P)，具有多个双层磷脂 分散体由以下物质制备 帝亚吉欧(1，2-dipalmitoyl-sn-glycero-3-phosphatidylcholine)一直被 用95 GHz的高分辨率EPR谱进行了研究。在一次 氮氧化物小探针3.3T EPR谱的共振场 2，2，6，6-四甲基-1-哌啶氧基(TEMPO) 水相和膜相被清楚地分解，进行测量 测头的动力学参数准确且毫不含糊。结果 表明尽管存在更亲油的造影剂， Gd-DOTA-P，可在双层内检测到，结构 膜的组织结构即使在 生理浓度高(5 Mm)。地球上的温度 双层膜的主相变也不受影响。 在其测定的0.4摄氏度的精度内。

Interactions of two MRI gadolinium contrast agents, gadolinium ethoxybenzyl-diethylenetriaminepentaacetate (Gd-EOB-DTPA) and gadolinium 2-pentyl-(1,4,7,10-tetraazacyclododecane-N, N', N'', N'''-tetraacetic acid) (Gd-DOTA-P), with multibilayer phospholipid dispersions prepared from 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC) have been investigated with high resolution EPR spectroscopy at 95 GHz. At a resonance field of 3.3 T EPR spectra of a small nitroxide probe, 2,2,6,6-tetramethyl-1-piperidinyloxy (Tempo), partitioned between aqueous and membrane phases are clearly resolved, making measurements of dynamics parameters of the probe accurate and unambiguous. Results show that although the presence of the more lipophilic contrast agent, Gd-DOTA-P, can be detected within the bilayer, the structural organization of the membrane remains unaffected even at physiologically high (5mM) concentrations. The temperature of the main phase transition of the bilayer was also unaffected to within the 0.4oC accuracy of its determination.