雌激素受体功能和核基质-猫眼课题宝

雌激素受体功能和核基质

批准号：

2900110

负责人：

DAVID L STENOIEN

金额：

$3.84万

依托单位：

BAYLOR COLLEGE OF MEDICINE

依托单位国家：

美国

项目类别：

财政年份：

1999

资助国家：

美国

项目状态：

未结题

起止时间：

1999-03-16 至

来源：

https://reporter.nih.gov/project-details/2900110

关键词：

HeLa cells MCF7 cell cell line confocal scanning microscopy estradiol estrogen receptors gene expression genetic transcription hormone regulation /control mechanism nuclear matrix point mutation polymerase chain reaction protein localization protein structure function tissue /cell culture transcription factor transfection

中文摘要

翻译

越来越多的人意识到核区隔是一种

英文摘要

There is a growing awareness that nuclear compartmentation is an essential component of nuclear metabolism. This proposal is aimed at studying the estrogen receptor as a model to understand how nuclear comparmentation affects transcriptional activity. To achieve this goal, the subnuclear domains targeted by ER under varying hormonal conditions will studied using high resolution microscopy and colocalization studies with other markers of specific subnuclear domains. The regions within the estrogen receptor responsible for interaction with the nuclear matrix will be analyzed by the generation of deletion constructs and chimeric proteins. Finally, the interaction of ER with transcriptional coactivators and regressors will be analyzed in a nucleoskeletal context to determine how subnuclear compartmentation of these factors regulates gene transcription.

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