IDENT AND POST-TRANSLATIONAL MODIFICATION OF MITOTIC REGULATORY PROTEINS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 随着蛋白质组学领域在识别细胞调控网络中的蛋白质组分方面取得了巨大的进展，理解这些蛋白质在细胞水平上是如何在空间和时间上进行调控的仍然是一个重要的任务。需要进行微创分析，以解决蛋白质在严格保持分隔化和代谢物浓度的自然环境中的功能。为了解决这些问题，我们建议开发和应用基于成像的技术来执行功能蛋白质组学，并评估细胞信号通路对完整细胞中蛋白质动力学的影响。这些技术将广泛应用于各种蛋白质网络的研究。为了开发这些方法，最初的实验将集中在氧化应激、辐射和细胞内信号通路对一类被称为染色体乘客的蛋白质的影响，这种蛋白质在细胞周期中经历了戏剧性的定位变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. As great progress is being made in the field of proteomics to identify the protein components in cell regulatory networks, understanding how these proteins are spatially and temporally regulated at the cellular level remains a significant task. Minimally invasive assays are required that can address how proteins function in their natural environment where compartmentalization and metabolite concentrations are strictly maintained. To address these issues, we propose to develop and apply imaging-based technologies to perform functional proteomics and assess the effects of cell-signaling pathways on protein dynamics in intact cells. These technologies will be broadly applicable to study a variety of protein networks. To develop these approaches, initial experiments will focus on the effects of oxidative stress, radiation, and intracellular signaling pathways on a class of proteins known as chromosomal passengers that undergo dramatic changes in localization during the cell cycle.