MOLECULAR NEUROGENETICS OF PHEROMONE PERCEPTION

信息素感知的分子神经遗传学

• 批准号: 2882683
• 负责人: ANDREA ROTHMAN
• 金额: $ 3.17万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2882683
• 关键词: animal communication behavior; animal genetic material tag; behavioral /social science research tag; gender difference; genetically modified animals; hormone receptor; laboratory mouse; neurogenetics; nucleic acid probes; nucleic acid sequence; olfactions; perception; pheromone; protein structure function; receptor binding; receptor expression; site directed mutagenesis; vomeronasal systems

Olfactory perception is mediated in mammals by two anatomically and functionally distinct sensory organs: the main olfactory epithelium (MOE) and the vomeronasal organ (VNO). While the MOE mediates detection of a wide variety of odorants, the VNO is responsible for recognition of pheromones. These are chemical signals that provide information about gender, dominance, or reproductive status. The recent isolation of a gene family encoding putative pheromone receptors provides a basis to study the molecular biology of pheromone perception. To date, however, there is no evidence that these proteins are pheromone receptors. Here, genetic proof is sought for the hypothesis that these genes encode molecules involved in pheromone perception. The applicant will attempt to identify a VNO receptor gene that is differentially expressed in the male or female, introduce a knock-out mutation in that gene, and assess the phenotype of the knock-out mice. The applicant will determine the ends of the cluster of VNO receptor genes, delete the whole gene family by chromosome engineering techniques, and assess the phenotype of the mutant mice.

嗅觉在哺乳动物中由两个解剖学上和 功能不同的感觉器官：主要的嗅觉上皮 (MOE)犁鼻器（vomeronasal organ，VNO） 而莫伊介导检测 在各种各样的气味中，VNO负责识别 费洛蒙 这些化学信号提供了 关于性别，支配地位，或者生育状况的问题 最近的隔离 一个基因家族编码假定的信息素受体提供了一个基础 来研究信息素感知的分子生物学 到目前为止， 然而，没有证据表明这些蛋白质是信息素 受体。在这里，遗传证据是为假设，这些 基因编码参与信息素感知的分子。 申请人 将试图确定一个VNO受体基因， 在雄性或雌性中表达，引入敲除突变， 基因，并评估敲除小鼠的表型。 申请人 将决定VNO受体基因簇的末端，删除 通过染色体工程技术，对基因家族进行了分析， 突变小鼠的表型。