MOLECULAR NEUROGENETICS OF PHEROMONE PERCEPTION

信息素感知的分子神经遗传学

• 批准号: 6164386
• 负责人: ANDREA ROTHMAN
• 金额: $ 3.75万
• 依托单位: ROCKEFELLER UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6164386
• 关键词: animal communication behavior; animal genetic material tag; behavioral /social science research tag; gender difference; genetically modified animals; hormone receptor; laboratory mouse; neurogenetics; nucleic acid probes; nucleic acid sequence; olfactions; perception; pheromone; protein structure function; receptor binding; receptor expression; site directed mutagenesis; vomeronasal systems

Olfactory perception is mediated in mammals by two anatomically and functionally distinct sensory organs: the main olfactory epithelium (MOE) and the vomeronasal organ (VNO). While the MOE mediates detection of a wide variety of odorants, the VNO is responsible for recognition of pheromones. These are chemical signals that provide information about gender, dominance, or reproductive status. The recent isolation of a gene family encoding putative pheromone receptors provides a basis to study the molecular biology of pheromone perception. To date, however, there is no evidence that these proteins are pheromone receptors. Here, genetic proof is sought for the hypothesis that these genes encode molecules involved in pheromone perception. The applicant will attempt to identify a VNO receptor gene that is differentially expressed in the male or female, introduce a knock-out mutation in that gene, and assess the phenotype of the knock-out mice. The applicant will determine the ends of the cluster of VNO receptor genes, delete the whole gene family by chromosome engineering techniques, and assess the phenotype of the mutant mice.

哺乳动物的嗅觉是由两个解剖学和 功能不同的感觉器官：主要嗅觉上皮 （MOE）和犁鼻器（VNO）。 当教育部调解检测时 VNO 负责识别多种气味剂 信息素。 这些是提供信息的化学信号 关于性别、统治地位或生殖状况。 最近的隔离 编码假定信息素受体的基因家族的研究提供了基础 研究信息素感知的分子生物学。 迄今为止， 然而，没有证据表明这些蛋白质是信息素 受体。在这里，我们寻求基因证据来证明这些假设 基因编码参与信息素感知的分子。 申请人 将尝试识别具有差异性的 VNO 受体基因 在男性或女性中表达，引入敲除突变 基因，并评估敲除小鼠的表型。 申请人 将确定 VNO 受体基因簇的末端，删除 通过染色体工程技术分析整个基因家族，并评估 突变小鼠的表型。