CONTROL OF SEX SPECIFIC NEURAL DEVELOPMENT AND BEHAVIOR

控制性别特异性神经发育和行为

• 批准号: 2891000
• 负责人: MICHAEL B MCKEOWN
• 金额: $ 27.97万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2891000
• 关键词: Drosophilidae; behavioral /social science research tag; developmental neurobiology; ethology; gender difference; neurogenetics; sex behavior

DESCRIPTION (Adapted from applicant's abstract): This proposal uses a newly discovered gene controlling multiple aspects of sexual behavior and neural development as an entry point to the dissection of the neurological, molecular and genetic mechanisms that generate of a set of complex behaviors in a higher organism. The dissatisfaction (dsf) gene of Drosophila controls appropriate sexual behavior in both males and females including sex partner choice, receptivity to courtship, copulation efficiency and egg laying behavior. It is also necessary for development of identified sex-specific neurons in both males and females. Genetic studies show that dsf defines a novel pathway that controls sexual differentiation of the nervous system but not of the cuticle. A combination of genetic, molecular, behavioral and neurological experiments will be used to investigate the mechanisms behind the generation of male- and female-specific behaviors. The structure and expression pattern of dsf will be used to gain insight into the mechanism by which dsf functions in the regulation of neural development. Studies of the temporal, spatial and sex-specific regulation of dsf function will be performed to understand integration of dsf into the general regulatory mechanisms functioning within the nervous system and into the genetic cascade which controls sexual development. The expression pattern of dsf and the projections of dsf-expressing cells will be mapped as part of the construction of a wiring diagram of the sex-specific nervous system. Various techniques will be used to study the specific defects that occur in both dsf-expressing and dsf-non-expressing cells in the absence of dsf function. A functional map of the sexual nervous system, both as it relates to general male and female behaviors and to the role of dsf, will be generated via the use of the regulatory sequences of dsf and other genes to target expression of potential key regulatory components to limited regions of the nervous system. Such "genetic sex-changes" will be coupled to behavioral analysis of animals whose non-nervous system sexual differentiation is genetically locked in a male or female mode, thus eliminating complications arising from intersexual or abnormal cuticular development. Genetic screens for genes which functionally interact with dsf will be initiated.

描述（改编自申请人摘要）：本提案使用了一种新的 发现了控制性行为和神经系统多个方面的基因 作为解剖神经系统的切入点， 产生一系列复杂行为的分子和遗传机制 在一个更高的有机体。 果蝇对照的不满足（dsf）基因 男性和女性的适当性行为，包括性伴侣 选择、求偶接受性、交配效率和产卵 行为 这也是必要的发展确定性别特异性 无论是男性还是女性。 遗传学研究表明，dsf定义了一个 控制神经系统性别分化的新途径， 而不是角质层 基因、分子、行为和 神经学实验将被用来研究背后的机制， 男性和女性特有行为的产生。 结构和 dsf的表达模式将被用来深入了解机制， DSF在神经发育的调节中起作用。 的研究 DSF功能时间、空间和性别特异性调节将 执行该操作以了解将dsf整合到一般监管中 神经系统内的功能机制，并进入遗传 控制性发育的级联反应。 dsf的表达模式 并且表达DSF的细胞的投影将被绘制为 构建性别特异性神经系统的接线图。 将使用各种技术来研究发生在 在不存在DSF的情况下表达DSF和不表达DSF的细胞 功能 性神经系统的功能图， 一般男性和女性的行为和dsf的作用，将是 通过使用DSF和其它基因的调节序列产生， 潜在关键调节成分在有限区域的靶向表达 的神经系统。 这种“基因性别变化”将与 非神经系统性行为动物的行为分析 分化在遗传上被锁定在男性或女性模式中，因此 消除由中间性或异常表皮引起的并发症 发展 与dsf功能性相互作用的基因的遗传筛选 将被启动。