STRUCTURAL DETERMINATION OF HK97 & CPMV

HK97是一种衣壳蛋白为650 A的dsDNA噬菌体。 大的单元电池需要使用最聪明和最高的 提供聚焦X射线束。我们已经收集了3.45A的数据 解析成功-在14-BM-C。这项实验的目的是 完成此分辨率的数据集。当前的完备性是 30%，分辨率在3.5-3.6A之间。APS数据在 香港97的结构决定。我们已经使用了这些数据 在1998年6月和8月收集，以将决议从5A延长到 3.8A.HK97晶体极其易碎，需要特殊的 正在处理。晶体直接在X射线毛细管中生长， 不能冻结。我们将在冷却到4度的情况下收集数据。C. 将使用距离为550 mm的Mar345探测器。一个 振荡角为0。曝光时间为18度 60秒。每个晶体平均曝光约10次。

HK97 is a dsDNA bacteriophage with a capsid of 650 A. The very large unit cell necessitates using the most brilliant and most highly focused x-ray beam available. We have collected data to 3.45 A resolution successfully-at 14-BM-C. The aim of this experiment is to complete the data set to this resolution. The current completeness is 30 % between 3.5 - 3.6 A resolution. The APS data has been pivotal in the HK97 structure determination. We have already used the data collected in June and August 1998 to extend the resolution from 5 A to 3.8 A. HK97 crystals are extremely fragile and require special handling. Crystals are grown directly in the X-ray capillary and cannot be frozen. We will collect data with cooling to 4 deg. C. The Mar345 detector will be used with a distance of 550 mm. An oscillation angle of 0. 18 degrees will be used with an exposure time of 60 seconds. Each crystal averages about 10 exposures.