FETAL VELOCIMETRY AND AMINO ACID TRANSPORT IN PREGNANCY

妊娠期的胎速测定和氨基酸转运

• 批准号: 2889331
• 负责人: FREDERICK C BATTAGLIA
• 金额: $ 14.85万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-20 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889331
• 关键词: acid base balance; aminoacid analyzer; aminoacid metabolism; aminoacid transport; blood chemistry; blood glucose; clinical research; gas chromatography mass spectrometry; high performance liquid chromatography; human pregnant subject; placental transfer; pregnancy; pregnancy circulation disorder; prenatal growth disorder; respiratory oxygenation; stable isotope diagnosis; statistics /biometry; ultrasound blood flow measurement; umbilical cord

The specific aims of this project involve clinical studies in normal pregnancies and pregnancies complicated by fetal growth restriction. The studies cover two aspects: 1) improvements in the classification of clinical severity using new fetal surveillance techniques and 2) studies of amino acid transport and metabolism using stable isotope methodology. The role of direct measurements of umbilical venous blood flow compared to arterial velocimetry measurements and fetal blood chemistry is examined around the framework of the classification of clinical severity. Similarly, the additional information acquired from single fetal organ velocimetry studies of the ductus venosus, fetal hepatic veins, fetal femoral artery, fetal middle cerebral artery are also compared to fetal umbilical arterial velocimetry and fetal chemistries. The studies of in vivo amino acid placental transport and metabolism is a follow up on our observations that fetal amino acid concentrations are significantly reduced in fetal growth restricted pregnancies. A non steady state study utilizing stable isoptomers of essential and non essential amino acids in pregnant women scheduled for cordocentesis examines in vivo transport for different groups of amino acids: a) to define differences in their relative rates of transport in vivo and b) to test whether amino acid transport is reduced in FGR pregnancies compared to normal pregnancies for all amino acids or a specific subset. Steady state stable isotope methodology is also used to test whether the fetal/maternal enrichment ratios for stable isoptomers of amino acids infused in the maternal circulation is lower in FGR pregnancies reflecting decreased transport and increased protein turnover. The relationship of maternal concentration to umbilical uptake for normal and FGR pregnancies is also investigated utilizing data on umbilical arteriovenous concentration differences at the time of cesarean section. All metabolic data are compared to the classification of clinical severity in FGR pregnancies using criteria we have previously published.

该项目的具体目标涉及正常情况下的临床研究 妊娠和妊娠并发胎儿生长受限。 研究涵盖两个方面：1）分类的改进 使用新的胎儿监测技术和 2) 研究的临床严重程度 使用稳定同位素方法研究氨基酸转运和代谢。 直接测量脐静脉血流量的作用比较 动脉测速测量和胎儿血液化学是 围绕临床严重程度分类的框架进行检查。 同样，从单个胎儿器官获得的附加信息 静脉导管、胎儿肝静脉、胎儿的测速研究 股动脉、胎儿大脑中动脉也与胎儿进行比较 脐动脉测速和胎儿化学。 的研究在 体内氨基酸胎盘转运和代谢是我们的后续 观察结果表明胎儿氨基酸浓度显着 胎儿生长受限妊娠减少。 非稳态 利用必需和非必需氨基的稳定异构体的研究 计划进行脐带穿刺术的孕妇体内酸含量 不同组氨基酸的运输：a) 定义差异 它们在体内的相对转运率和 b) 测试氨基是否 与正常情况相比，FGR 妊娠中的酸转运减少 所有氨基酸或特定子集的妊娠。 稳态稳定 同位素方法也用于测试胎儿/母体是否 注入氨基酸的稳定异构体的富集率 FGR 妊娠期间母体循环较低，反映出 FGR 减少 运输和增加蛋白质周转。 母系关系 正常妊娠和 FGR 妊娠的脐带摄取浓度也 利用脐动静脉浓度数据进行研究 剖腹产时的差异。 所有代谢数据均 与 FGR 妊娠临床严重程度分类的比较 使用我们之前发布的标准。