CENTRAL CONTROL OF MOTONEURONS IN A VERTEBRATE

脊椎动物运动神经元的中央控制

• 批准号: 2908363
• 负责人: JOSEPH R. FETCHO
• 金额: $ 1.95万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2908363
• 关键词: Mauthner's neuron; chordate locomotion; diving /swimming; electrophysiology; escape reaction; goldfish; intercellular connection; interneurons; motor neurons; neural information processing; neuromuscular system; neurophysiology; psychomotor function; reticulospinal tract; single cell analysis; spinal cord mapping; synapses

Movements are produced by activity in populations of neurons, but little is known about the patterns of recruitment in neuronal populations and their relationships to behavior. This proposal outlines studies of how different forms of a simple vertebrate behavior-the escape behavior in zebrafish-are produced by activity in pools of hindbrain reticulospinal neurons and spinal interneurons. The work uses powerful new imaging techniques that take advantage of the transparency of larval zebrafish and allow the imaging of activity in cells in the intact animal, as well as the photoablation of cells to study the resulting behavior changes. The aims are: 1) to use photoabalations to test the hypothesis that the hindbrain contains serially repeated sets of functionally related reticulospinal neurons that act as a population to produce escape turns of different magnitudes; 2) To use photoablations to provide the first direct test of the functional role of at a class of spinal interneuron in a behavior; 3) To use calcium imaging to study how the spinal interneurons in a population are recruited during movements (escapes) of different strength and; 4) To use ablations, combined with imaging of activity, to study the links between reticulospinal activity and spinal interneuron recruitment. The work is of general importance because many of the descending commands for movement from the brain are channeled through the reticulospinal system and spinal interneurons, but we know little about the patterns of activity in these neuronal populations and their contributions to behavior. Each of the proposed aims not only provides a piece of the information needed for better understanding of one behavior, they also address fundamental issues in the control of movement that will apply broadly among vertebrates. These include the functional significance of hindbrain segmentation, the behavioral role of spinal interneurons, patterns of interneuron recruitment, and the links between reticulospinal neurons, premotor interneurons and behavior. The work is basic research dealing with principals of motor organization. The establishment of the principals by which normal movements are produced provides the foundation for understanding the disruptions of movement that occur in various diseases. The work is of additional significance because of the increasing importance of zebrafish as at a model system in development and genetics. The power of the eventual combination of development, genetics, functional imaging and behavioral studies make the further development of this model system especially important.

运动是由神经元群体的活动产生的，但很少 已知神经元群体的募集模式， 它们与行为的关系。 该提案概述了如何研究 不同形式的一个简单的脊椎动物行为-逃避行为， 斑马鱼-是由活动池后脑网状脊髓 神经元和脊髓中间神经元。 这项工作使用了强大的新成像技术 利用斑马鱼幼鱼透明性的技术 也可以对完整动物的细胞活动进行成像， 因为光消融细胞来研究由此产生的行为变化。 目的是：1）利用光烧蚀来检验假设， 后脑包含一系列重复的功能相关的 网状脊髓神经元作为一个群体产生逃避转向 2）使用光消融提供第一个 一类脊髓中间神经元功能作用的直接测试 3）使用钙成像来研究脊髓如何 群体中的中间神经元在运动（逃逸）期间被招募 4）采用消融术，结合影像学检查 研究网状脊髓活动与 脊髓中间神经元募集 这项工作具有普遍的重要性 因为大脑发出的许多下行运动指令 通过网状脊髓系统和脊髓中间神经元，但 我们对这些神经元的活动模式知之甚少， 人口及其对行为的贡献。 每个拟议 aims不仅提供了更好地 了解一种行为，他们还解决了基本问题， 这种运动控制将广泛应用于脊椎动物。 这些包括后脑分割的功能意义， 脊髓中间神经元的行为作用，中间神经元的模式 募集，以及网状脊髓神经元之间的联系，运动前 interneurons神经元and behavior行为.这项工作是基础研究， 运动组织的负责人。 校长的设立 产生正常运动的基础， 了解发生在各种不同的运动中断， 疾病 这项工作具有额外的意义，因为 斑马鱼作为发展中的模型系统的重要性日益增加 和遗传学。 最终结合发展的力量， 遗传学、功能成像和行为研究使得 这个模型系统的开发尤为重要。