CENTRAL CONTROL OF MOTONEURONS IN A VERTEBRATE

脊椎动物运动神经元的中央控制

• 批准号: 3477555
• 负责人: JOSEPH R. FETCHO
• 金额: $ 9.34万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1993-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3477555
• 关键词: Mauthner's neuron; central nervous system; diving /swimming; electrophysiology; escape reaction; fresh water environment; goldfish; histology; interneurons; motor neurons; neural information processing; neural inhibition; neural initiation; spinal nerves; synapses

One of the primary goals of studies of the motor systems of vertebrates is to understand how the central nervous system controls the activation of motoneurons to generate different motor behaviors. The networks underlying motor behaviors are most easily studied in fish and amphibians because of the smaller number and larger size of their neurons relative to those in mammals. The proposed experiments are designed to examine the spinal circuitry that controls different populations of axial motoneurons during two very different motor behaviors in goldfish - escapes initiated by the Mauthner cell and normal swimming. Intracellular recording and HRP staining techniques will be used to: 1) examine the output connections of identified interneurons in the spinal network of the M-cell. These data will provide essential information about the role of identified interneurons in the network of a well defined vertebrate motor behavior. 2) determine what neurons are polysynaptically activity by the M- cell. These data will permit a detailed comparison between the activation of motoneurons and interneurons by the M-cell network and the swimming network. 3) determine what spinal interneurons and motoneurons are active during fictive swimming in goldfish, as a basis for comparison with both the M-cell network and the spinal network for swimming on other vertebrates. 4) determine directly what synaptic inputs individual interneurons receive from both networks to evaluate the extent to which spinal interneurons are shared by the two. 5) examine interactions between the swimming and M-cell networks by eliciting a M-cell initiated escape during fictive swimming episode. These experiments will provide basic information about the central mechanisms for controlling the activation of axial motoneurons in goldfish. Because previous work indicates broad similarities in the organization of spinal motor systems in vertebrates, the results are likely to provide general insights into mechanisms for control of motoneurons in vertebrates, including humans.

研究运动系统的主要目标之一 脊椎动物是要了解中枢神经系统如何 控制运动神经元的激活以产生不同的电动机 行为。 电机行为的基础网络最容易 由于数量较小，在鱼类和两栖动物中学习 相对于哺乳动物的神经元的大小较大。 这 建议的实验旨在检查脊柱回路 在两个过程中控制不同的轴向运动神经元种群 金鱼中的运动行为截然不同 - 逃脱由 Mauthner细胞和正常游泳。 将使用细胞内记录和HRP染色技术 到： 1）检查已确定的中间神经元的输出连接 M细胞的脊柱网络。 这些数据将提供必不可少的 有关确定的中间神经元在 定义明确的脊椎动物运动行为的网络。 2）确定哪些神经元是通过M-进行多突触活动的 细胞。 这些数据将允许在 M细胞网络激活运动神经元和中间神经元 和游泳网络。 3）确定哪些脊柱中间神经元和运动神经元有效 在虚构的金鱼游泳期间，作为与之比较的基础 M-Cell网络和脊柱网络均可游泳 其他脊椎动物。 4）直接确定什么突触输入单个神经元 从两个网络接收以评估脊柱的程度 中间神经元由两者共享。 5）检查游泳和M细胞网络之间的相互作用 通过引起虚拟游泳期间的M细胞逃脱 插曲。 这些实验将提供有关中央的基本信息 控制轴向运动神经元激活的机制 金鱼。 因为以前的工作表明了广泛的相似之处 脊椎动物中脊柱运动系统的组织， 结果可能会提供对机制的一般见解 控制包括人类在内的脊椎动物中的运动神经元。