STRESS ACTIVATED PROTEIN KINASE IN NEURONAL APOPTOSIS

神经元凋亡中的应激激活蛋白激酶

• 批准号: 2750983
• 负责人: PASKO RAKIC
• 金额: $ 29.14万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2004-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2750983
• 关键词: Parkinson's disease; amyotrophic lateral sclerosis; apoptosis; calcium flux; cerebral ischemia /hypoxia; enzyme activity; enzyme induction /repression; genetically modified animals; gerbil /jird; glutamates; isozymes; laboratory mouse; laboratory rat; methylphenyltetrahydropyridine; nervous system transplantation; neural degeneration; neurotoxicology; neurotoxins; oxidative stress; protein kinase; stress proteins; superoxide dismutase; tissue /cell culture; transplant rejection; xenotransplantation

Glutamate excitotoxicity is the presumed mechanism underlying many neurological disorders including acute ischemia and chronic neurodegeneration. Recent work from our laboratories demonstrated that mice lacking the neural isoform of the stress activated protein kinsase SAPK/JNK (JNK3) has remarkable resistance to kainic acid-induced seizures, AP-1 transcriptional activity and apoptosis of hippocampal neurons. These results strongly suggest that the SAPK/JNK signaling pathway is a critical component in the pathogenesis of glutamate excitotoxicity. Based on this hypothesis, the present proposal comprises two sets of closely related in vitro and in vivo experiments. Specific Aim I: Mechanism of JNK -mediated glutamate neurotoxicity ( in vitro studies). Keys issues to be addressed include: (1) the JNK - mediated neurotoxicity through gene regulation and de novo biosynthesis; (2) the regulation of intracellular calcium oscillation through JNK signaling; (3) the interplay between JNK - signaling And oxidative stress, (4) the specificity of activation route of JNK signaling. These potential mechanisms will be tested in primary culture of dissociated neurons using a combination of morphological, physiological and biochemical approaches. Specific Aim II: Neuroprotection of the blockade JNK signaling pathway (in vivo studies) Potential clinical applications of inhibition of the JNK signaling may include: (1) the prevention of ischemic apoptosis; (2) attenuation of chronic glutamate excitotoxicity in amytrophic lateral sclerosis; (3) the prevention of neurodegneration (4) enhanced survival of grafted neurons in transplantation therapy. Preliminary evidence of feasibility has been obtained for each Specific Aim. The potential applications will be tested in experimental models of neuropathology using wildtype and mutant mice lacking the neural-specific JNK3 isoform. In summary, the overall goal of the present proposal is both to understand the mechanism underlying JNK- mediated glutamate neurotoxicity and to test the clinical potential of targeting the JNK signaling pathway for therapeutic intervention.

谷氨酸兴奋毒性是许多神经系统疾病包括急性缺血和慢性神经变性的潜在机制。 本实验室最近的工作表明，缺乏应激激活蛋白激酶SAPK/JNK（JNK 3）神经亚型的小鼠对红藻氨酸诱导的癫痫发作、AP-1转录活性和海马神经元凋亡具有显著的抵抗性。 这些结果有力地表明，SAPK/JNK信号通路是谷氨酸兴奋毒性的发病机制中的一个关键组成部分。 基于这一假设，本提案包括两组密切相关的体外和体内实验。具体目标I：JNK介导的谷氨酸神经毒性机制（体外研究）. 其中需要解决的关键问题包括：（1）JNK通过基因调控和从头生物合成介导的神经毒性;（2）JNK信号转导对细胞内钙振荡的调控;（3）JNK信号转导与氧化应激的相互作用;（4）JNK信号转导激活途径的特异性。 这些潜在的机制将在原代培养的解离神经元使用形态学，生理学和生化方法的组合进行测试。 具体目标二：阻断JNK信号通路的神经保护作用（体内研究）抑制JNK信号通路的潜在临床应用可能包括：（1）预防缺血性细胞凋亡;（2）减弱肌萎缩侧索硬化症中的慢性谷氨酸兴奋毒性;（3）预防神经变性;（4）提高移植治疗中移植神经元的存活率。 每个具体目标的可行性初步证据已经获得。 潜在的应用将在神经病理学实验模型中使用缺乏神经特异性JNK 3亚型的野生型和突变型小鼠进行测试。总之，本提案的总体目标是了解JNK介导的谷氨酸神经毒性的潜在机制，并测试靶向JNK信号传导途径用于治疗干预的临床潜力。