SODIUM FLUORIDE, METHYLEUGENOL, & ANTHRAQUINONE

该项目的目标是确定潜在的毒性和 氟化钠、甲基亮酚和任选的致癌性 经口给Fischer 344大鼠和B6C3F1小鼠。这个 每种化学品的基本奖项包括分析技术的发展 化学和剂量公式方法。一旦这些方法被 应进行充分开发的毒性/致癌性研究。 氟化钠--将进行为期104周的研究，涉及 雄性大鼠，按3个剂量加水灌胃，临时 在26周和65周时牺牲。将在临时阶段进行特别研究 牺牲包括血液学；血清钙、磷、氟和 碱性磷酸酶；和氟化骨。血清和骨氟 浓度是在终极祭祀时测量的。全身 所有动物在尸检前都要拍X光片。 学习。 甲基丁香酚--首先要进行毒代动力学研究 建立基本的毒物动力学参数，以确定 生物蓄积和确定实际的内照射剂量 曝光。将进行一项为期104周的研究，涉及50名- 动物/性别/物种，3次剂量加灌胃对照。额外的60 每个性别的大鼠都要接受为期26周的一次剂量的停止研究。 每只雌性增加10只大鼠，在26周时被处死 其余的将继续寻求014只接受车辆治疗。 毒代动力学研究将在慢性研究期间进行，以 评价长期接触和年龄对毒物动力学的影响 参数。 可选研究--将进行为期13周的研究 涉及10只动物/性别/物种，5个剂量，外加剂量饲料的对照。 特别研究包括血液学、临床化学和尿液分析。 大鼠，小鼠的血液学；大鼠和小鼠的SMVCE；毒代动力学研究 以建立基本的毒物动力学参数，以确定 生物蓄积的程度和确定实际的内照射剂量 一次曝光。 将进行为期104周的研究，涉及50只动物/性别/物种， 3暴露浓度加对照。毒物动力学研究将是 在慢性研究期间进行，以评估长期治疗的效果 暴露和年龄对毒物动力学参数的影响。

The objective of this project is to determine the potential toxicity and carcinogenicity of sodium fluoride, methyleugenol, and optionally anthraquinone, by oral routes to Fischer 344 rats and B6C3F1 mice. The basic award for each chemical includes the development of analytical chemistry and dose formulation methods. Once these methods have been adequately developed, toxicity/carcinogenicity studies are to be conducted. Sodium fluoride -- 104-week study is to be conducted, involving seventy male rats, at 3 doses plus controls via dosed water, with interim sacrifices at 26 and 65 weeks. Special studies to be conducted at interim sacrifices include hematology; serum calcium, phosphorus, fluoride and alkaline phosphatase; and bone fluoride. Serum and bone fluoride concentrations are to be measured at terminal sacrifice. Whole body radiographs are to be made for all animals prior to necropsy throughout the study. Methyleugenol -- Toxicokinetic studies are to be conducted first to establish basic toxicokinetic parameters, to determine the extent of bioaccumulation and to determine actual internal doses after a single exposure. A 104-week study is to be conducted, involving fifty- animals/sex/species, at 3 doses plus controls via gavage. An additional 60 rats per sex are to be treated for 26 weeks at one dose for a stop study. Ten of these additional rats per sex are to be sacrificed at 26 weeks and the remainder will continue to seek 014 treated with vehicle only. Toxicokinetic studies are to be conducted during the chronic study to evaluate the effects of long term exposure and age on toxicokinetic parameters. Optional Study of Anthraquinone -- A 13-week study is to be conducted, involving ten animals/sex/species, at 5 doses plus controls via dosed feed. Special studies include hematology, clinical chemistries and urinalysis in rats, hematology in mice; SMVCE in rats and mice; toxicokinetic studies are to be conducted to establish basic toxicokinetic parameters, to determine the extent of bioaccumulation and to determine actual internal doses after a single exposure. 104-week study is to be conducted, involving fifty animals/sex/species, at 3 exposure concentrations plus controls. Toxicokinetic studies are to be conducted during the chronic study to evaluate the effects of long term exposure and age on toxicokinetic parameters.