COLONIC CYTOKINETICS AND CELL SIGNALING--DIETARY EFFECT
结肠细胞动力学和细胞信号转导——饮食效应
基本信息
- 批准号:2895009
- 负责人:
- 金额:$ 25.98万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1994
- 资助国家:美国
- 起止时间:1994-12-23 至 2003-04-30
- 项目状态:已结题
- 来源:
- 关键词:DNA repair adduct antisense nucleic acid apoptosis athymic mouse biological signal transduction carcinogenesis inhibitor cell differentiation cell growth regulation cell line chemoprevention colon neoplasms dietary lipid enzyme activity enzyme inhibitors immunocytochemistry intestinal mucosa isozymes laboratory rat neoplastic transformation nutrition aspect of cancer nutrition related tag omega 3 fatty acid phenotype protein kinase C
项目摘要
Among dietary factors, there is strong epidemiological, clinical and
experimental data indicating a protective effect on n-3
polyunsaturated fatty acids (n-3 PUFAs; eicosapentaenoic acid, 20:5n-
3 and docosahexaenoic acid, 22:n-3) on colon cancer. We have
recently demonstrated that dietary n-3 PUFAs confer protection
against experimental carcinogenesis, i.e., a reduction in tumor
incidence, in part by enhancing the deletion of cells through
activation of apoptosis, which may reduce the accumulation of genetic
errors. These data support our postulate that dietary n-3 PUFAs act
as anticarcinogens by facilitating the apoptotic removal of
carcinogen adducted cells. In order to further elucidate the
mechanism(s) by which n-3 PUFAs-induce apoptosis. We will utilize the
highly relevant rat model of colon carcinogenesis to determine
whether n-3 PUFAs modulate DNA adduct formation, removal (DNA repair)
and/or deletion (apoptosis) during the initial stages of malignant
transformation. We have also recently show that n-3 PUFAs prevent
the carcinogen-induced chronic down-regulation of colonic protein
kinase C (PKC) delta (novel), zeta (atypical), and the selective up-
regulation of PKC betaII (classical). This is significant because
the maintenance of crypt PKC levels may sustain the homeostatic
balance between cell proliferation and apoptosis. Therefore, we have
hypothesized that n-3 PUFAs reduce colon cancer incidence in part by
blocking the effects of carcinogen on colonic PKC isozyme-related
signal transduction. To further determine the significance of n-3
PUFA-induced changes in colonic PKC expression, we will elucidate the
role of specific PKC isozymes in colon tumor development by using a
targeted pharmacological inhibitor in vivo in combination with
overepression and antisense strategies in vitro. Elucidation of the
mechanism(s) by which dietary n-3 PUFAs reduce colon cancer incidence
will lead to the establishment of dietary guidelines designed to
reduce colon cancer morbidity and mortality. This experimental
approach is particularly relevant because despite advancement in the
treatment of colon cancer, the 5 year mortality rate has not
appreciable improved over the past 4 decades. Therefore,
chemopreventive dietary strategies must be developed in order to
decrease the risk of colon cancer.
在饮食因素中,有很强的流行病学,临床和
表明对N-3有保护作用的实验数据
多不饱和脂肪酸(n-3 PUFA;二十碳五烯酸,20:5 n-
3和二十二碳六烯酸,22:n-3)对结肠癌的作用。 我们有
最近证明,膳食n-3多不饱和脂肪酸提供保护,
对抗实验性致癌作用,即,减小肿瘤
发病率,部分是通过增强细胞的缺失,
细胞凋亡的激活,这可能会减少遗传物质的积累,
错误. 这些数据支持了我们的假设,即膳食n-3 PUFA
作为抗癌剂,通过促进细胞凋亡去除
致癌物内收细胞。 为了进一步阐明
n-3 PUFA诱导细胞凋亡的机制。我们将利用
高度相关的大鼠结肠癌模型,以确定
n-3 PUFA是否调节DNA加合物的形成、去除(DNA修复)
和/或缺失(凋亡)在恶性肿瘤的初始阶段
转型 我们最近还表明,n-3 PUFA可以防止
致癌物诱导结肠蛋白慢性下调
激酶C(PKC)δ(新),ζ(非典型)和选择性上调-
PKC β II的调节(经典)。 这一点意义重大,因为
维持隐窝PKC水平可能维持体内平衡,
细胞增殖和凋亡之间的平衡。 所以我们
假设n-3 PUFA降低结肠癌发病率的部分原因是
阻断致癌物对结肠PKC同工酶的影响
信号转导 为了进一步确定n-3的意义
PUFA诱导的结肠PKC表达变化,我们将阐明
用免疫组织化学方法研究特异性PKC同工酶在结肠肿瘤发生中的作用
体内靶向药理学抑制剂与
体外的反义抑制和反义策略。 阐明
饮食n-3 PUFA降低结肠癌发病率的机制
将导致建立膳食指南,
降低结肠癌发病率和死亡率。 该实验
这种方法特别重要,因为尽管在
结肠癌的治疗,5年死亡率没有
在过去的四十年里,有了明显的改善。 因此,我们认为,
必须制定化学预防饮食策略,
降低患结肠癌的风险。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Robert Stephen Chapkin其他文献
Robert Stephen Chapkin的其他文献
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