PROTEIN INTERACTIONS AND REPRESSION FUNCTION OF E1A

描述：E1 A相关蛋白，p300，是主要的兴趣， 癌症生物学家，因为作为转化功能的直接靶点， 在腺病毒E1 A基因中，它参与了两种细胞的调节， 周期特异性和组织特异性基因表达。 P300是一种高 与CREB辅激活因子CBP同源。 CREB和CBP是主要的 介导的转录反应的环AMP反应途径。 p300和CBP之间的关系表明，它们的表现相似， 功能协调发展的 尽管如此，它们是由不同的基因编码的，并且 初步临床证据表明，特定的代谢紊乱 与CBP的选择性丢失有关，表明p300和CBP不是 功能冗余。 研究人员发现了一种新的270千道尔顿的 与p300和CBP相关的产品。 p270特别是 有趣的是，它存在于细胞中，作为一种紧密的 相关的蛋白质系列。 此外，他们还确定了一个突出的 BRG 1是这个复合体的成员。 BRG 1蛋白质产品是一种 转录激活因子，一种基因表达途径的调节因子， 与发育和分化，和一个推定的DNA解旋酶。 的 p207/BRG 1复合物具有完整的这种活性。 这个的鉴定 互动应该打开理解的主要部分， p300和BRG 1家族的分子作用机制，两个家族 这些蛋白质似乎是基因表达的关键调节因子， 控制细胞增殖和分化的途径。

DESCRIPTION: The E1A-associated protein, p300, is of major interest to cancer biologists because, as a direct target of the transforming functions of the adenovirus E1A gene, it is implicated in the regulation of both cell cycle specific and tissue specific gene expression. p300 is highly homologous to the CREB coactivator, CBP. CREB and CBP are the major mediators of the transcriptional response to cyclic AMP-responsive pathways. The relationship between p300 and CBP suggests that they perform, similar functions. Nevertheless, they are encoded by different genes, and preliminary clinical evidence indicates that specific metabolic disorders are linked with selective loss of CBP, indicating that p300 and CBP are not functionally redundant. The investigators identified a new 270 kilodalton product that is related to both p300 and CBP. p270 is particularly interesting because it exists in cells as a component of a tightly associated series of proteins. Moreover they have identified a prominent member of this complex as BRG1. The BRG1 protein product is a transcriptional activator, a regulator of gene expression pathways linked with the development and differentiation, and a putative DNA helicase. The p207/BRG1 complexes with this activity intact. The identification of this interaction should open the way to understanding a major part of the molecular mechanisms of action of the p300 and BRG1 families, two families of proteins that appear to be pivotal regulators of gene expression in pathways controlling cell proliferation and differentiation.