FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
基本信息
- 批准号:2897093
- 负责人:
- 金额:$ 10.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-04-01 至 2001-03-31
- 项目状态:已结题
- 来源:
- 关键词:SDS polyacrylamide gel electrophoresis adenosinetriphosphatase antibody biopsy cytoskeletal proteins electron microscopy gene expression human tissue immunocytochemistry in situ hybridization major histocompatibility complex mastication muscle function muscle metabolism muscle tension myofibrils myosins polymerase chain reaction protein isoforms sarcomeres systematic biology
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Mammalian skeletal
muscle fibers have been traditionally classified into specific types by
characterization of their myofibrillar ATPase and metabolic
histochemistry and more recently by immunohistochemistry with myosin
heavy chain (MHC)-specific antibodies. Human skeletal muscle contains
three main fiber types: I, IIA and IIX (or IIB) derived from specific
MHC isoforms. Mammalian jaw-closing muscle fibers are notably different
in comparison to other skeletal muscle, reflecting the specialized nature
of their functions. The jaw-closing muscle fibers of the human primate
are the most exceptional, expressing developmental and cardiac MHC and
myosin light chain (MLC) isoforms not found in other adult skeletal
muscle, as well as the typical adult skeletal isoforms. A full
description of myosin expression in human masseter is presently
incomplete, and a fiber type classification scheme, incorporating both
myosin protein content and contractile function, is lacking. Using human
masseter muscle biopsies, the first specific aim of this study is to
develop a fiber type classification scheme for jaw-closing muscles based
on MHC and MLC identification as determined by tissue staining for:
myofibrillar ATPase and metabolic histochemistry, immunohistochemistry
with MHC-specific antibodies and in situ hybridization with probes
developed from different MHC gene transcripts. In whole muscle biopsies
and in single fibers, MHC content will be confirmed by glycerol-SDS-
PAGE, and MLC content by 2-D electrophoresis. In addition, electron
microscopy will be used to evaluate the relative heterogeneous
distribution of myosin isoforms within representative fibers. The second
aim of this study is to determine the maximum speed of unloaded
shortening (Vo), as determined by the slack test, and specific tension
(Po) of the individual chemically skinned or freeze-dried fibers
representative of each fiber type. Following physiology, the relative
percent content of MHC and MLC isoforms in these single fibers will also
be correlated to Vo and Po to determine if these physiologic parameters
are related to contractile protein expression as is the case in other
human skeletal muscle. The long-term objectives of this work are to
provide detailed, basic information regarding the exceedingly complex
contractile protein expression in human jaw-closing muscle, the
classification of specific fiber types distinct or similar to other
skeletal muscle, and the main physiological properties of each fiber
type. This information may serve as a basis from which dentistry may
apply a more thorough understanding of jaw-closing muscle function to
common clinical problems such as temporomandibular joint disorders,
abnormal craniofacial growth and stability after orthognathic surgical
procedures.
描述(改编自申请人摘要):哺乳动物骨骼
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('JAMES J SCIOTE', 18)}}的其他基金
Musculoskeletal Heritable Influences on Malocclusion
肌肉骨骼遗传对咬合不正的影响
- 批准号:
8536253 - 财政年份:2012
- 资助金额:
$ 10.19万 - 项目类别:
Musculoskeletal Heritable Influences on Malocclusion
肌肉骨骼遗传对咬合不正的影响
- 批准号:
8385400 - 财政年份:2012
- 资助金额:
$ 10.19万 - 项目类别:
FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
- 批准号:
6176944 - 财政年份:1996
- 资助金额:
$ 10.19万 - 项目类别:
FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
- 批准号:
2133103 - 财政年份:1996
- 资助金额:
$ 10.19万 - 项目类别:
FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
- 批准号:
2684007 - 财政年份:1996
- 资助金额:
$ 10.19万 - 项目类别:
FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
- 批准号:
2133104 - 财政年份:1996
- 资助金额:
$ 10.19万 - 项目类别:
FUNCTIONAL AND MOLECULAR STUDY OF HUMAN MASSETER FIBERS
人体咬肌纤维的功能和分子研究
- 批准号:
2391232 - 财政年份:1996
- 资助金额:
$ 10.19万 - 项目类别:
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