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RECOGNITION OF HIV INFECTED CELLS BY CYTOTOXIC T CELLS

细胞毒性 T 细胞对 HIV 感染细胞的识别

基本信息

批准号：
2886048
负责人：
Kathleen L. Collins
金额：
$ 9.23万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-01 至 2001-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2886048
关键词：
HIV infections alkaline phosphatase biomarker blood chemistry cell line cell population study clinical research clone cells cytotoxic T lymphocyte flow cytometry helper T lymphocyte human immunodeficiency virus 1 human subject recombinant virus virus infection mechanism

项目摘要

The candidate is particularly interested in understanding the mechanism by which Hiv evades immune surviellance. An increased understanding of how this occurs may be critical for the development chemotherapeutics and vaccines. In the proposed research, cytotxic t lymphocytes (cTLs) isolated from Hiv postive patients will be tested for their ability tokill primary CD4 postive cell isolates infected with HIV. Career Devleopment Plan: Dr. collins will have no specific training activityes aside from the performance of research. She will participate in weekly laboratory meetings encompassing a broad range of research activities, as well as journal club and periodic scientific meetings. She will be encouraged to attend scientific seminars presented at MIT and surrounding institutes. Research Plan: the research plan takes a phased approach, starting with Hiv-1 infected t-cell lines and cloned DC8 cells. Then primary cells will be infected, purified, and used as targets. CD8 positive cells from Hiv-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 postive individuals will be engineered to express a selectable marker so that infected cells can be purified.

候选人特别感兴趣的是了解艾滋病毒逃避免疫存活的机制。一个增加对这一现象如何发生的理解可能对开发化疗药物和疫苗。在建议的从HIV阳性患者中分离出细胞毒T淋巴细胞(CTL)的研究患者将接受测试，以确定他们是否有能力杀死原发的CD4阳性患者感染艾滋病毒的细胞分离株。职业发展计划：柯林斯博士不会接受任何专门的培训除了研究的表现外，还有其他活动。她会的参加每周的实验室会议，包括广泛的研究活动的范围，以及期刊俱乐部和定期科学会议。她将被鼓励参加科学课程在麻省理工学院和周边机构举办的研讨会。研究计划：研究计划采取分阶段的方法，从用HIV-1感染的T细胞系和克隆的DC8细胞。然后原代细胞将被感染、纯化并用作靶点。CD8 来自HIV-1阳性个体的阳性细胞将被取代了CD8克隆。最后，内源性病毒来自 HIV-1阳性的人将被取代CD8 克隆人。最后，来自HIV-1阳性个体的内源性病毒将被改造成表达一种可选择的标记，以便感染细胞可以被提纯。