RECOGNITION OF HIV INFECTED CELLS BY CYTOTOXIC T CELLS

应聘者特别有兴趣了解 HIV逃避免疫存活的机制。 一个 增加对这种情况如何发生的理解可能是至关重要的 开发化疗药物和疫苗。 拟议 研究，从HIV阳性患者中分离出的细胞毒性T淋巴细胞（CTL） 将检测患者杀死原发性CD 4阳性细胞的能力 感染艾滋病毒的细胞分离物。 职业发展计划：柯林斯博士将没有具体的培训 活动是的，除了进行研究之外。 她将 参加每周一次的实验室会议， 研究活动的范围，以及期刊俱乐部和定期 科学会议。 她将被鼓励参加科学 在麻省理工学院和周边研究所举办的研讨会。 研究计划：研究计划采取分阶段的方法，从 用HIV-1感染的T细胞系和克隆的DC 8细胞。 然后 原代细胞将被感染、纯化并用作靶。 CD8 然后将来自HIV-1阳性个体的阳性细胞 取代了CD 8克隆。 最后，来自 HIV-1阳性个体将取代CD 8 克隆 最后，来自HIV-1阳性个体的内源性病毒 将被工程化以表达选择性标记， 可以纯化细胞。

The candidate is particularly interested in understanding the mechanism by which Hiv evades immune surviellance. An increased understanding of how this occurs may be critical for the development chemotherapeutics and vaccines. In the proposed research, cytotxic t lymphocytes (cTLs) isolated from Hiv postive patients will be tested for their ability tokill primary CD4 postive cell isolates infected with HIV. Career Devleopment Plan: Dr. collins will have no specific training activityes aside from the performance of research. She will participate in weekly laboratory meetings encompassing a broad range of research activities, as well as journal club and periodic scientific meetings. She will be encouraged to attend scientific seminars presented at MIT and surrounding institutes. Research Plan: the research plan takes a phased approach, starting with Hiv-1 infected t-cell lines and cloned DC8 cells. Then primary cells will be infected, purified, and used as targets. CD8 positive cells from Hiv-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 positive individuals will then be substituted for the CD8 clones. Finally, endogenous viruses from HIV-1 postive individuals will be engineered to express a selectable marker so that infected cells can be purified.