REGULATION OF ICTACALCIN GENE EXPRESSION

伊卡钙素基因表达的调控

• 批准号: 2737396
• 负责人: ANGELA R PORTA
• 金额: $ 8.17万
• 依托单位: KEAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-02-01 至 2002-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2737396
• 关键词: DNA footprinting; biological signal transduction; calcium binding protein; calcium flux; catfish; gel mobility shift assay; gene expression; genetic library; genetic promoter element; genetic regulation; molecular cloning; nucleic acid sequence; olfactory lobe; transcription factor

DESCRIPTION (adapted from applicant's abstract): Ictacalcin is a calcium-binding protein that is found in olfactory receptor neurons in the catfish. This AREA proposal describes a series of experiments aimed at characterizing the ictacalcin gene and the mechanisms underlying its restricted expression to olfactory neurons. Olfactory signal transduction in vertebrates, mediated through G protein coupled pathways, results eventually in fluctuations of cytosolic calcium in the cilia and soma of receptor neurons. Thus, calcium is thought to be involved in signal generation, adaptation and/or termination of the sensory signal. Ictacalcin belongs to the S100 family of calcium binding proteins, the members of which may play a role in mediating the effects of calcium in a variety of other tissues. Because of its tissue-specific expression in the olfactory epithelium, the regulation of the ictacalcin gene is potentially interesting. In this application, Dr. Porta proposes to isolate the full-length ictacalcin gene and initiate a definition of transcriptional elements in its regulatory regions and of cell type specific transcriptional factors involved in its regulation. In the long-term, the P.I. believes that this work has significance for understanding the regulation of chemosensory transduction and other neuronal processes.

描述（改编自申请人的摘要）：衣钙素是一种 钙结合蛋白，存在于嗅觉受体神经元中 鲶鱼该区域提案描述了一系列旨在 在描述ictacalcin基因及其潜在机制方面， 限制性表达于嗅觉神经元。嗅觉信号 在脊椎动物中，通过G蛋白偶联介导的转导 通路，最终导致细胞内钙离子的波动， 感受神经元的纤毛和索马。因此，钙被认为是 参与信号生成、适配和/或终止 感觉信号Ictacalcin属于钙结合的S100家族 蛋白质，其成员可能在介导效应中起作用 其他组织中的钙含量。由于其组织特异性 在嗅上皮中的表达， 基因可能很有趣。在这个应用程序中，Porta博士建议 分离出完整的ictacalcin基因， 在其调控区和细胞类型的转录元件 参与其调控的特异性转录因子。在 从长远来看，私家侦探认为这项工作对 了解化学感觉传导的调节和其他 神经突起