YOLK SAC STEM CELL DIFFERENTIATION IN VITRO AND VIVO

描述（改编自研究者摘要）： 该应用是体外扩增的卵黄囊造血干细胞， 可以成功地移植到组织不相容的宿主中， 分化，从而建立免疫功能。 具体目标是 1）建立促进卵黄囊衍生的自我更新的条件 体外培养小鼠造血干细胞; 2）测定小鼠造血干细胞的能力， 扩增的卵黄囊衍生的干细胞群（a）以接种鼠靶器官 在组织相容性宿主动物中;（B）产生各种血细胞 谱系;和（c）产生能够在免疫系统中起作用的细胞。 系统;以及3）确定扩增的卵黄囊来源的干细胞是否 群体也可以播种小鼠靶器官并在 组织不相容宿主动物。

DESCRIPTION (Adapted from the investigator's abstract): The hypothesis of this application is that in vitro expanded yolk sac hematopoietic stem cells can successfully engraft in histoincompatible hosts in which they then differentiate, thereby establishing immune functions. The specific aims are to: 1) establish conditions that promote self-renewal of yolk sac-derived mouse hematopoietic stem cells in vitro; 2) determine the ability of the expanded yolk sac-derived stem population (a) to seed murine target organs in histocompatible host animals; (b) to generate the various blood cell lineages; and (c) to yield cells capable of functioning in the immune system; and 3) determine whether the expanded yolk sac-derived stem cell population can also seed murine target organs and differentiate in histoincompatible host animals.