ORIGIN OF LYMPHOCYTES IN THE EMBRYONIC OMENTUM

这项研究旨在确定发展中的 小鼠胚胎早期网膜原基形成的潜力 淋巴细胞 初步研究表明， 早期小鼠胚胎含有能形成淋巴细胞的干细胞， 拟议的研究工作的目的是描述 网膜来源的细胞，并确定它们的发育起源和 它们在免疫功能中的后续作用。 具体的实验目的包括：1）分析形成的淋巴样细胞 通过移植或体外培养13天小鼠网膜原基， （分化标记，凝集素结合特性，器官特异性 抗原;体内外功能研究;集落形成 潜力）; 2）大网膜的组织发生和形态发生研究 从小鼠发育到出生的第10天的原基，包括体内和 干细胞群的体外表征;和3）研究 大网膜雏形的发展与遗传损伤的 造血（W系列），内脏中胚层（Dh/+，Dh/Dh），胸腺 (nu/nu）或B细胞成熟（CBA/Nxid）系统。 这项研究的基本目标是研究一种新的 胚胎淋巴细胞，以获得更好的理解， 淋巴系统的个体发育，并希望分析 大网膜来源的淋巴细胞可能定义了一个特定的， 在胎儿和/或成人免疫功能中重要的细胞亚群。

The proposed research is directed at determining the developmental potential of the early omental rudiment of the mouse embryo to form lymphocytes. Preliminary studies indicate that the omental anlage of the early mouse embryo contains stem cells that can form lymphocytes, and it is the purpose of the proposed research work to characterize the omentum-derived cells and to determine both their developmental origin and their subsequent role in immune functions. Specific experimental aims include: 1) Analysis of lymphoid cells formed by the 13-day mouse omental rudiment in transplants or in vitro (differentiation markers, lectin-binding properties, organ-specific antigens; functional studies in vivo and in vitro; colony forming potential); 2) study of histogenesis and morphogenesis of the omental anlage from day 10 of mouse development to birth, including the in vivo and in vitro characterization of stem cell populations; and 3) investigation of the development of omental rudiments with genetic impairment of the hematopoietic (W series), splanchnic mesodermal (Dh/+,Dh/Dh), thymic (nu/nu) or B-cell maturation (CBA/N xid) systems. The basic goal of the proposed research is to study a new source of embryonic lymphocytes in an effort to gain greater understanding of the ontogeny of lymphoid systems in general, and in the hope that the analysis of omentum-derived lymphocytes may define a specific and possibly new subset of cells important in fetal and/or adult immune functions.