EFFECTS OF ZINC OXIDE WELDING FUME INHALATION

流感样综合征的发烧，肌痛，呼吸系统的投诉，所谓的 “金属烟雾热”与吸入氧化锌烟雾有关。 尽管在那些暴露的人中熟悉这种常见的情况， 在职业卫生保健提供者中， 其原因机制，氧化锌烟雾剂量反应 关系，以及疾病的预防和治疗。 我 假设氧化锌烟雾引起细胞因子的释放， 肺，这些细胞因子作为内源性热原和趋化性 炎症反应的因素。 我提议检验这个假设 并为观察到的效应生成详细的剂量反应数据， 镀锌低碳钢电弧焊研究课题 在一个环境暴露室里。 我会测量肺功能 包括基线时、1小时时支气管对乙酰甲胆碱的反应性 暴露后20小时。 我会进行支气管肺泡灌洗 (BAL)在暴露后22小时和2至4个月之间再一次。 我 将分析BAL的总细胞计数和分类计数，并测量 ELISA法检测白细胞介素1（IL-1）和肿瘤坏死因子（TNF）。 我会 还测量BAL细胞中IL-1的mRNA。我会把这些 氧化锌暴露数据的生理和细胞效应 通过个人呼吸区监测和血锌采集 程度. 锌介导的金属烟雾热是一组职业性 疾病，包括由于聚合物和有机粉尘吸入引起的综合征， 提示共同的病理生理机制。 本研究探讨了 肺和全身综合征是一个重要的临床问题， 职业医学确定与细胞因子的关联， 描述氧化锌烟雾的剂量反应将有助于 了解金属烟雾热等的原因及预防 类似的职业病。

The flu-like syndrome of fever, myalgia, and respiratory complaints called "metal fume fever" is associated with inhalation of zinc oxide fume. Despite familiarity with this common condition among those exposed and among occupational health care providers, little is known about the mechanisms of its causation, the zinc oxide fume dose response relationship, and the prevention and treatment of the disorder. I hypothesize that zinc oxide fume causes the release of cytokines in the lung and that these cytokines act as endogenous pyrogens and chemotactic factors for an inflammatory response. I propose to test this hypothesis and to generate detailed dose response data for the effects observed by studying subjects performing electric arc welding on galvanized mild steel in an environmental exposure chamber. I will measure pulmonary function, including bronchial responsiveness to methacholine, at baseline, 1 hour and 20 hours after exposure. I will.carry out bronchoalveolar lavage (BAL) at 22 hours and once again between 2 to 4 months after exposure. I will analyze BAL for total cell count and differential and measure interleukin 1 (IL-1) and tumor necrosis factor (TNF) by ELISA. I will also measure mRNA for IL-1 in the BAL cells. I will correlate these physiologic and cellular effects with exposure data for zinc oxide collected by personal breathing zone monitoring and with blood zinc levels. Zinc mediated metal fume fever is one of a group of occupational disorders, including syndromes due to polymer and organic dust inhalation, suggesting common pathophysiologic mechanisms. This study addresses a pulmonary and systemic syndrome that is an important clinical problem in occupational medicine. Identifying an association with cytokines while delineating the dose response to zinc oxide fume will contribute to an understanding of the causes and prevention of metal fume fever and other similar occupational syndromes.