EFFECTS OF ZINC OXIDE WELDING FUME INHALATION

氧化锌焊接烟雾吸入的影响

• 批准号: 3068972
• 负责人: Paul D Blanc
• 金额: $ 5.39万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-28 至 1993-09-27
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3068972
• 关键词: alveolar macrophages; diagnostic respiratory lavage; engine exhaust; human subject; hyperthermia; industrial waste; industry; interleukin 1; lung; metal oxide; occupational disease /disorder; occupational hazard; pyrogens; respiratory function; respiratory toxin; smoke inhalation; tumor necrosis factor alpha; zinc

The flu-like syndrome of fever, myalgia, and respiratory complaints called "metal fume fever" is associated with inhalation of zinc oxide fume. Despite familiarity with this common condition among those exposed and among occupational health care providers, little is known about the mechanisms of its causation, the zinc oxide fume dose response relationship, and the prevention and treatment of the disorder. I hypothesize that zinc oxide fume causes the release of cytokines in the lung and that these cytokines act as endogenous pyrogens and chemotactic factors for an inflammatory response. I propose to test this hypothesis and to generate detailed dose response data for the effects observed by studying subjects performing electric arc welding on galvanized mild steel in an environmental exposure chamber. I will measure pulmonary function, including bronchial responsiveness to methacholine, at baseline, 1 hour and 20 hours after exposure. I will.carry out bronchoalveolar lavage (BAL) at 22 hours and once again between 2 to 4 months after exposure. I will analyze BAL for total cell count and differential and measure interleukin 1 (IL-1) and tumor necrosis factor (TNF) by ELISA. I will also measure mRNA for IL-1 in the BAL cells. I will correlate these physiologic and cellular effects with exposure data for zinc oxide collected by personal breathing zone monitoring and with blood zinc levels. Zinc mediated metal fume fever is one of a group of occupational disorders, including syndromes due to polymer and organic dust inhalation, suggesting common pathophysiologic mechanisms. This study addresses a pulmonary and systemic syndrome that is an important clinical problem in occupational medicine. Identifying an association with cytokines while delineating the dose response to zinc oxide fume will contribute to an understanding of the causes and prevention of metal fume fever and other similar occupational syndromes.

发烧、肌痛和呼吸道疾病的流感样综合征称为 “金属烟雾热”与吸入氧化锌烟雾有关。 尽管对暴露者和患者中的这种常见情况很熟悉， 在职业卫生保健提供者中，人们对职业卫生保健知之甚少 其因果机制，氧化锌烟雾剂量反应 关系以及疾病的预防和治疗。 我 假设氧化锌烟雾导致细胞因子的释放 肺，这些细胞因子充当内源性热原和趋化剂 炎症反应的因素。 我建议检验这个假设 并为所观察到的效应生成详细的剂量反应数据 研究在镀锌低碳钢上进行电弧焊的科目 在环境暴露室中。 我会测量肺功能， 包括支气管对乙酰甲胆碱的反应性，基线，1小时 以及暴露后20小时。 我会进行支气管肺泡灌洗 (BAL) 在 22 小时内进行一次，并在暴露后 2 至 4 个月内再次进行一次。 我 将分析 BAL 的总细胞计数和差异并进行测量 通过 ELISA 检测白细胞介素 1 (IL-1) 和肿瘤坏死因子 (TNF)。 我会 还测量 BAL 细胞中 IL-1 的 mRNA。我会将这些关联起来 氧化锌暴露数据的生理和细胞效应 通过个人呼吸区监测和血锌收集 水平。 锌介导的金属烟雾热是一组职业性热病之一 疾病，包括由于吸入聚合物和有机粉尘引起的综合症， 提示共同的病理生理机制。 这项研究解决了一个 肺部和全身综合征是一个重要的临床问题 职业医学。确定与细胞因子的关联 描述氧化锌烟雾的剂量反应将有助于 了解金属烟雾热等的原因和预防 类似的职业综合症。