ANIMAL RESOURCES IMPROVEMENT

儿童医院奥克兰研究所(CHORI)是一家私立、 基础和临床生物医学研究的非营利性组织 在各种学科中进行，如血液学，生物化学， 免疫学、遗传学、癌症、肺部发育和细胞生物学。这个 CHORI动物资源设施(ARF)安置和维护传统小鼠 和老鼠，偶尔还有兔子，用作实验动物。在……里面 此外，一群免疫受损的突变SCID/SCID小鼠 最近成立的。机构动物保护和利用委员会 (IACUC)确定了ARF中的几个主要缺陷，其中包括： 没有单独的区域来处理干净和肮脏的笼子、瓶子等， “干净”和“肮脏”的交通流之间没有区分，不充分 材料储存和分离，缺乏气压控制 单独的动物房间和无法控制空气温度 独立的隔间，以及不完善的电气系统，如没有 应急电气系统(也控制暖通空调)和不变灯 动物房间里的紧张气氛。另外，单独的程序室不是 目前可用于对动物进行实验性操作。 这项建议的目的是纠正这些缺陷，以便 提供促进动物健康和福祉的环境条件， 确保有效的动物管理程序，并提供设施 有利于动物实验。另一个目标是升级 ARF足以遵守美国国立卫生研究院关于护理和使用 并获得AAALAC认证。要实现这些 目标，描述了一个对ARF物理装置进行改造的项目：1) 翻新现有的笼子/瓶子加工区，使独立的 为搬运和清洁笼子、瓶子、 等2)通过以下方式提高笼子和瓶子消毒程序的质量 安装笼式/瓶式洗瓶器和高压灭菌器，3)改进 需要安装单独的暖通空调的环境控制系统 动物宿舍系统、变温装置和 光强系统和应急备用电源系统的提供 以及4)翻新现有的实验室，以提供单独的动物 与ARF相邻的程序室。对CHORI的这些修改 ARF将促进良好的畜牧业实践，从而改善动物 健康和设施管理。

Children's Hospital Oakland Research Institute (CHORI) is a private, non-profit organization where basic and clinical biomedical research is conducted in a variety of disciplines such as hematology, biochemistry, immunology, genetics, cancer, pulmonary development and cell biology. The CHORI Animal Resource Facility (ARF) houses and maintains conventional mice and rats and occasionally rabbits, for use as experimental animals. In addition, a colony of immunologically compromised mutant scid/scid mice was recently established. The Institutional Animal Care and Use Committee (IACUC) has identified several major deficiencies in the ARF which include: lack of separate areas for processing clean and dirty cages, bottles, etc., lack of separation between "clean" and "dirty" traffic flows, inadequate material storage and segregation, lack of air pressure control to individual animal rooms and inability to control air temperature in individual cubicles, and inadequate electrical systems such as absence of emergency electrical system (also controls HVAC) and invariant light intensity in animal rooms. Also, a separate procedures room is not currently available for performing experimental manipulations of animals. The objective of this proposal is to correct these deficiencies so as to provide environmental conditions which promote animal heath and well-being, ensure efficient animal management procedures, and provide facilities conducive to animal experimentation. An additional goal is to upgrade the ARF sufficiently to comply with NIH guidelines for the care and use of laboratory animals and to obtain AAALAC certification. To realize these objectives, a project is described to modify the ARF physical plant by: 1) renovating an existing cage/bottle processing area such that separate utility areas are created for the handling and cleaning of cages, bottles, etc. 2) improving the quality of cage and bottle sanitizing procedures by installing a cage/bottle washer and autoclave, 3) improving the environmental control systems which entails installation of a separate HVAC system for the animal quarters, installation of variable temperature and light intensity systems and provision of an emergency power back up system and 4) renovating an existing laboratory to provide a separate animal procedures room contiguous with the ARF. These modifications to the CHORI ARF will promote good animal husbandry practices and thus improve animal health and facilities management.