ACELLULAR VACCINES AGAINST BACTERIAL PATHOGENS

这项研究的直接目标是构建一个有效的、 百日咳杆菌感染的无毒无细胞疫苗。 百日咳毒素是由百日咳杆菌产生的一种胞外蛋白。 既是毒力的决定因素，又是一种引起保护性的抗原 豁免权。将建立产生一种类毒素的条件 一种无细胞毒性但具有免疫原性的百日咳毒素。这个 基因工程百日咳毒素类毒素将单独进行测试，并 与百日咳杆菌的其他抗原一起作为无细胞疫苗候选。这个 长远目标是制定战略，建设一个 百日咳杆菌无细胞疫苗适用于 重组DNA源无细胞疫苗的研制 细菌！病原体。 所描述的研究项目将定义 百日咳毒素的S1亚基。其目的是定义的功能 S1亚基内降低毒性但保留的特定残基 免疫原性。这项研究还将重点确定 从百日咳毒素的定义探讨百日咳毒素的作用机制 被激活，进入敏感细胞，并与G蛋白相互作用。

The immediate goal of this research is to construct an effective, nontoxic acellular vaccine against infection by Bordetella pertussis. Pertussis toxin is an extracellular protein produced by B. Pertussis that is both a determinant of virulence and an antigen that elicits protective immunity. Conditions will be established to produce a toxoid of pertussis toxin that lacks cytotoxicity but retains immunogenicity. The genetically engineered toxoid of pertussis toxin will be tested alone and with other antigens of B. pertussis as acellular vaccine candidates. The long-term goals are to develop strategies for the construction of an acellular vaccine against B. pertussis that are applicable for the generation of recombinant DNA-derived acellular vaccines against other bacterial !pathogens. The research project described will define the catalytic properties of the Sl subunit of pertussis toxin. The aim is to define the function of specific residues within the Sl subunit that reduce toxicity but retain immunogenicity. This research will also focus on determining the mechanism of action of pertussis toxin by defining how pertussis toxin is activated, enters sensitive cells, and interacts with G-proteins.