ACTIVATION AND CONTROL OF RRNA TRANSCRIPTION

RRNA 转录的激活和控制

• 批准号: 3070983
• 负责人: Richard L. Gourse
• 金额: $ 5.61万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3070983
• 关键词: DNA binding protein; DNA directed RNA polymerase; DNA footprinting; Escherichia coli; RNA biosynthesis; bacterial genetics; genetic promoter element; genetic transcription; mutant; nucleic acid sequence; protein biosynthesis; ribosomal RNA; ribosomes; transcription factor

In E. coli, the synthesis of ribosomes is controlled primarily at the stage of rRNA transcription initiation. rRNA can account for as much as 50% of instantaneous cellular RNA synthesis. In order to understand the biosynthesis of the protein synthetic apparatus, factors involved in the upstream activation and growth rate dependent regulation of rRNA synthesis are being investigated. The mechanism of rRNA promoter-RNA polymerase association is being studied using kinetic and footprinting methods. This will lead to the identification of the individual steps involved in transcription initiation at the rrnB PL promoter and the factors that influence each step. The specific DNA sequences required for upstream activation and growth rate control will be defined by construction and selection of site mutations, followed by assay of operon fusions in vivo. Finally, the importance of position, orientation, and conformation of the upstream activator sequence (UAS) relative to the promoter and the feasibility of using the UAS to enhance transcription from heterologous promoters will also be examined using operon fusions in vivo.

在大肠杆菌中，核糖体的合成主要受控于 rRNA转录起始阶段。 rRNA 可以解释为 高达瞬时细胞 RNA 合成的 50%。 为了 了解蛋白质合成装置的生物合成， 影响上游激活和增长率的因素 rRNA 合成的依赖性调节正在研究中。 rRNA启动子-RNA聚合酶联合的机制是 正在使用动力学和足迹方法进行研究。 这将 导致识别所涉及的各个步骤 rrnB PL 启动子处的转录起始及其因子 影响每一步。 所需的特定 DNA 序列 上游激活和增长率控制将由下式定义 位点突变的构建和选择，然后进行分析 体内操纵子融合。 最后再说一下职位的重要性， 上游激活子序列的方向和构象 （UAS）相对于发起人以及使用的可行性 UAS 增强异源启动子的转录将 还可以使用体内操纵子融合进行检查。