MEDCHANISM, ACTIVATION, & CONTROL OF RRNA TRANSCRIPTION

机制、激活、

• 批准号: 3291940
• 负责人: Richard L. Gourse
• 金额: $ 18.22万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3291940
• 关键词: DNA directed RNA polymerase; DNA footprinting; Escherichia coli; RNA biosynthesis; bacterial genetics; gene expression; genetic manipulation; genetic promoter element; genetic transcription; mutant; nucleic acid sequence; nucleic acid structure; operon; protein biosynthesis; ribosomal RNA; ribosomes; solutions

In E. coli, the synthesis of ribosomes is controlled primarily at the stage of rRNA transcription initiation. rRNA can account for as much as 50% of instantaneous cellular RNa synthesis. In order to understand the biosynthesis of the protein synthetic apparatus, factors involved in the upstream activation and growth rate dependent regulation of rRNA synthesis are being investigated. The mechanism of rRNA promoter-RNa polymerase asociation is being studied using kinetic and footprinting methods. This will lead to the identification of the individual steps involved in transcription initiation at the rrnB P1 promoter and the factors that influence each step. The specific DNA sequences required for upstream activation and growth rate control will be defined by construction and selection of site mutations, followed by assay of operon fusions in vivo. Finally, the importance of position, orientation, and conformation of the upstream activator sequence (UAS) relative to the promoter and the feasibility of using the UAS to enhance transcription from heterologous promoters will also be examined using operon fusions in vivo.

在大肠杆菌中，核糖体的合成主要在这一阶段受到控制