B LYMPHOCYTE ACTIVATION

当前的目标是描绘出 功能上重要的分子如Lyb 2、Ia和BSF-1 受体参与B淋巴细胞活化。 长期 目标是设计出调节B细胞功能的方法， 低免疫状态以及定义的生化基础， B细胞的生长和分化， 生物化学、细胞免疫学和分子生物学。 本研究的长期目标是了解 免疫异常的潜在基础 年龄 最初，研究的重点将是功能角色 两种B细胞表面分子Lyb 2和Ia。 战略将 用来克隆Lyb 2抗原的基因 一个概念是， 一种独特的B细胞亚群参与对 将评价多糖抗原。 B 细胞亚群在生长或成熟因子方面可能不同， 它们表达的受体将通过制备单克隆抗体来研究。 这些B细胞表面分子的抗体。 生化和 来自老年小鼠的B细胞的表型异常将被 研究了 年轻人和成年人的B细胞库的差异 将通过定量以下的表达来确定老年小鼠： 小鼠重链可变区基因家族的核酸分析 酸杂交技术。 这些研究应该使我们能够更好地了解B细胞的生长 并制定更好的战略， 免疫调节

The immediate goals are to delineate the mechanisms by which functionally important molecules such as Lyb2, Ia and BSF-1 receptors participate in B lymphocyte activation. Long term goals are to devise ways to regulate B cell function in hyper- and hypo-immune states as well as to define the biochemical basis of B cell growth and differentiation using the approaches of biochemistry, cellular immunology and molecular biology. The long term objectives of this research are to understand the underlying basis for the immune abnormalities associated with age. Initially, the focus of the research will be the functional role of two B cell surface molecules Lyb2 and Ia. Strategies will be developed to clone the gene for Lyb2 antigen. The concept that a unique B cell subset is involved in antibody responses to polysaccharide antigens will be evaluated. The possibility that B cell subsets may differ in the growth or maturation factor receptors they express will be studied by preparing monoclonal antibodies to such B cell surface molecules. Biochemical and phenotypic abnormalities in B cells from aged mice will be studied. The repertoire differences in B cells from young and aged mice will be determined by quantitating the expression of the murine heavy chain variable region gene families by nucleic acid hybridization techniques. These studies should enable us to better understand B cell growth and differentiation and to develop better strategies for immunomodulation.