AUTORADIOGRAPHY & PHYSIOLOGY OF GASTROINTESTINE PEPTIDES

该研究计划由两个主要项目组成。第一个是 确定猫回盲括约肌的生理反应 (ICS)进食和局部刺激，如扩张，以及 确定解剖学的各个组成部分的贡献 对这些生理反应的反应。这些研究将有助于 了解ICS的综合作用(如果有的话) 回肠和结肠的不同功能。该项目 包括使用三种方法，一种体内方法和两种体内方法 用体外方法检查这些问题。存在不一致之处 体内和体外研究之间的文献。这个 建议的研究将用于确定出现这种情况的原因。 差异。介导ICS松弛的通路 近端扩张和远端扩张的收缩 已定义。许多被释放的多肽的作用 在用餐后，或被描述为出现在ICS中，以及 它们的作用部位将在体内和体外进行检测。这个 回肠、ICS和结肠在该区域的综合功能将 在体内和体外使用肌切开术进行检查。在……里面 此外，血管活性肠肽可能作为 神经递质介导的ICS松弛将被研究。 这项研究计划的第二个主要项目是开发 多肽受体放射自显影技术的研究进展 胃肠道。放射自显影的方法学研究 阿片类、P物质、蛙皮素类，以及胆碱能和 肾上腺素能受体将被确定。这项技术并没有 以前被应用于胃肠道方面 相关的神经肽和神经递质 胃肠动力。定量放射自显影将允许 不同地区受体位置和密度的比较 并允许比较胃肠道的 体内和体内具有生理反应的受体群体 体外培养。

The research plan consists of two major projects. The first is to define the physiologic responses of the feline ileocecal sphincter (ICS) to feeding and to local stimuli such as distension, and to determine the contribution of various components of the anatomic ICS to these physiologic responses. These studies will contribute to an understanding of the integrative role, if any, of the ICS to the differing functions of the ileum and colon. The project consists of using three methods, an in vivo method and two in vitro methods to examine these problems. Discrepancy exists in the literature between studies performed in vivo and in vitro. The proposed studies will be directed to determine the reason for such discrepancies. The pathways mediating ICS relaxation to proximal distension, and contraction to distal distension will be defined. The effect of many peptides which are either released after meals or have been described to be present in the ICS, and their sites of action will be examined in vivo and in vitro. The integrative function of the ileum, ICS and colon in this area will be examined using myotomy techniques in vivo and in vitro. In addition, the possible role of vasoactive intestinal peptide as the neurotransmitter mediating relaxation at the ICS will be studied. The second major project of this research plan is the development of the technique of autoradiography of peptide receptors in the gastrointestinal tract. The methodology for autoradiography of opiate-, substance P-, bombesin-, as well as cholinergic and adrenergic receptors will be determined. This technique has not previously been applied to the gastrointestinal tract with respect to neuropeptides and neurotransmitters relevant to gastrointestinal motility. Quantitative autoradiography will allow the comparison of receptor sites and densities in different areas of the gastrointestinal tract and allow a comparison of the receptor population with physiologic responses in vivo and in vitro.