FOLLICULAR GROWTH AND ATRESIA

卵泡生长和闭锁

• 批准号: 3073165
• 负责人: ANNE N HIRSHFIELD
• 金额: $ 5.76万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-09-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3073165
• 关键词: autoradiography; basement membrane; cell differentiation; corpus luteum; estrus; follicle stimulating hormone; graafian follicles; histology; hormone regulation /control mechanism; menstrual cycle disorder; ovary disorder; radioimmunoassay

The long-term objective of this research is to understand the regulation of ovarian function at the tissue level, by defining the mechanisms which permit one follicle to complete the process of follicular development and ovulate, while relegating others to atresia, before development is completed. The experiments in this proposal focus on the role of FSH in the selection process. The specific aims of this project are: 1) To define the mechanism by which elevated FSH recruits follicles by monitoring rates of granulosa cell proliferation, incidence of atresia, and evidence of cell differentiation following manipulation of endogenous FSH concentrations. 2) To characterize the feedback relationship between FSH and early antral follicles during the selection process on estrus by a) altering the number of early antral follicles or the concentration of FSH in the circulation on estrus and determining if these interventions prevent the appearance of atretic follicles in the intact rat on metestrus, and b) comparing the selection process in intact rats with that in log-term hemicastrate rats, where selection occurs without atresia. 3) To test the hypothesis that changes in the composition of the basement membrane are involved in the mechanisms of atresia by using immunohistochemical techniques to examine changes in the molecular composition of the basal lamina during the selection of early antral follicles on estrus. 4) To determine the mechanism by which the corpus luteum of the mentrual cycle inhibits follicular development in the rhesus monkey, by monitoring proliferation rates of granulosa cells and the incidence of atresia following luteectomy in different size classes of follicles.

这项研究的长期目标是了解 在组织水平上的卵巢功能，通过定义 允许一个卵泡完成卵泡发育过程 排卵，而将其他人降级到闭锁，在发育之前 完成。这一方案中的实验重点是FSH在 选拔过程。该项目的具体目标是： 1)明确升高的FSH通过以下方式招募卵泡的机制 监测颗粒细胞增殖率、闭锁发生率和 内源性FSH处理后细胞分化的证据 浓度。 2)刻画FSH与早期胃窦的反馈关系 发情期选择过程中的卵泡，通过a)改变数量 早期有腔卵泡的数量或循环中FSH浓度 发情，并确定这些干预措施是否防止出现 正常大鼠动情后期的闭锁卵泡，以及b)比较 完整大鼠的选择过程与对数足月半原肠大鼠的选择过程相同， 在没有闭锁的情况下进行选择。 3)检验基底成分变化的假设 膜在闭锁机制中的作用 检测分子变化的免疫组织化学技术 早期上颌窦选择过程中基板的组成 发情期的毛囊。 4)确定胚胎黄体形成的机制。 周期通过监测抑制恒河猴卵泡发育 颗粒细胞增殖率与闭锁发生率的关系 不同大小卵泡的黄体切除后。