FOLLICLE SELECTION: A CELL KINETIC STUDY

卵泡选择：细胞动力学研究

• 批准号: 3314542
• 负责人: ANNE N HIRSHFIELD
• 金额: $ 5.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-03-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3314542
• 关键词: autoradiography; cell cycle; cell differentiation; cell population study; estrus; follicle stimulating hormone; graafian follicles; hormone regulation /control mechanism; hyperplasia; luteinizing hormone; oogenesis; ovariectomy; ovulation; progesterone; radioimmunoassay; radiotracer

The early antral stage is a pivotal point in follicular development in the cyclic rat. Follicles leave this stage by only two paths: some go on to mature in preparation for ovulation; all others degenerate (undergo atresia). Although the FSH surge plays a major role in determining which follicles will ovulate, its precise mechanism of actin remains unclear. The research proposed here will test the hypothesis that high concentrations of FSH are required during the early antral stage of development to initiate differentiation of the granulosa cells. Because the process of differentiation is associated with the end of cell division, the dynamics of cell proliferation should change as the selection process takes place. I will: (1) fully characterize the dynamics of granulosa cell growth using techniques of cell cycle analysis to identify this decision point in follicular development. (2) measure several parameters of granulosa cell proliferation (growth fraction, birth rate, generation time, synchrony) and determine serum FSH concentrations to correlate cyclic variation in FSH secretion with changes in granulosa cell growth rates. (3) experimentally alter endogenous FSH concentrations (with porcine follicular fluid or hemicastration) to directly test the hypothesis that FSH regulates granulosa cell proliferation. These studies will begin to define the precise mechanism by which one follicle is chosen for ovulation while another is relegated to astresia.

胃窦早期是卵泡发育的关键点。 循环大鼠。毛囊离开这个阶段只有两条路：有些继续到 成熟的为排卵做准备的；所有其他的退化的(经历 闭锁)。尽管卵泡刺激素激增在决定 卵泡会排卵，但其肌动蛋白的确切机制尚不清楚。 这里提出的研究将检验这一假设，即高 在胃窦早期需要FSH浓度。 发育以启动颗粒细胞的分化。因为 分化的过程与细胞分裂的结束有关， 随着选择过程的进行，细胞增殖的动态也应该改变 发生了。我将：(1)充分描述颗粒的动力学 使用细胞周期分析技术来鉴定细胞生长 卵泡发育的决定点。(2)测量几个参数 颗粒细胞增殖(生长分数、出生率、世代 时间，同步)和测定血清FSH浓度以关联循环 卵泡刺激素分泌随颗粒细胞生长速率的变化而变化。 (3)实验改变内源性FSH浓度(与猪 卵泡液或半去势)，以直接检验假设 FSH对颗粒细胞的增殖有调节作用。这些研究将开始 确定选择一个卵泡进行排卵的精确机制 而另一个则被降级到了阿斯特雷西亚。