MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS

胆碱能神经元的分子生物学

• 批准号: 3078396
• 负责人: STEPHEN A BERMAN
• 金额: $ 5.72万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3078396
• 关键词: Alzheimer's disease; antibody; autoradiography; brain cell; cell death; cell growth regulation; choline acetyltransferase; gene expression; genetic library; genetic manipulation; genetic recombination; human tissue; messenger RNA; molecular cloning; molecular pathology; monoclonal antibody; nucleic acid sequence; placenta; polysomes; postmortem; prosencephalon; radiotracer

Monoclonal and polyclonal antibodies to choline acetyl transferase (ChAT) will be used to immunoisolate messenger RNA which codes for the ChAT enzyme. Human placental tissue, which has been shown to be a rich source of ChAT, will be used as a source of polysomes for this effort. Human tissue (obtained by autopsies of Alzheimer's disease patients as well as controls who died of other causes) from the basal forebrain regions will be utilized to isolated mRNA so that disease specific and region specific libraries of complementary DNA can be created. These libraries will be used to screen for cDNA clones whose presence or absence is associated with Alzheimer's disease. The ChAT clones and the other Alzheimer related clones will be used to study the growth and development of brain cells in culture via in situ hybridization and RNA quantitation.

胆碱乙酰转移酶(ChAT)的单抗和多克隆抗体 将被用来免疫分离信使RNA，它编码聊天 酵素。人类胎盘组织，已被证明是一个丰富的来源 聊天，将被用作这一努力的多聚体来源。人类 组织(通过阿尔茨海默病患者的尸检获得)以及 对照谁死于其他原因)将从基底前脑区域 用于分离mRNA，以便特定于疾病和特定地区 可以创建互补DNA文库。这些库将是 用于筛选其存在或缺失与以下各项相关的cdna克隆 阿尔茨海默氏症。聊天克隆和其他与阿尔茨海默氏症相关的人 克隆将被用来研究脑细胞的生长和发育 通过原位杂交和RNA定量进行培养。