MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS

胆碱乙酰转移酶（ChAT）的单克隆和多克隆抗体 将用于免疫分离编码ChAT的信使RNA 酵素 人类胎盘组织，已被证明是一个丰富的来源， 的ChAT，将被用作该努力的多聚核糖体的来源。 人类 组织（通过阿尔茨海默病患者的尸检获得， 控制谁死于其他原因）从基底前脑区域将是 用于分离mRNA，使得疾病特异性和区域特异性 可以创建互补DNA文库。 这些图书馆将 用于筛选cDNA克隆，其存在或不存在与 老年痴呆症 ChAT克隆和其他阿尔茨海默病相关的 克隆将被用于研究脑细胞的生长和发育， 通过原位杂交和RNA定量进行培养。

Monoclonal and polyclonal antibodies to choline acetyl transferase (ChAT) will be used to immunoisolate messenger RNA which codes for the ChAT enzyme. Human placental tissue, which has been shown to be a rich source of ChAT, will be used as a source of polysomes for this effort. Human tissue (obtained by autopsies of Alzheimer's disease patients as well as controls who died of other causes) from the basal forebrain regions will be utilized to isolated mRNA so that disease specific and region specific libraries of complementary DNA can be created. These libraries will be used to screen for cDNA clones whose presence or absence is associated with Alzheimer's disease. The ChAT clones and the other Alzheimer related clones will be used to study the growth and development of brain cells in culture via in situ hybridization and RNA quantitation.