MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
批准号:
3078398
负责人:
STEPHEN A BERMAN
金额:
$5.72万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1985
资助国家:
美国
项目状态:
已结题
起止时间:
1985-04-01 至 1990-03-31
关键词:
Alzheimer's disease antibody autoradiography brain cell cell death cell growth regulation chick embryo choline acetyltransferase gene expression genetic library genetic manipulation genetic recombination human tissue in situ hybridization laboratory rat messenger RNA molecular cloning molecular pathology monoclonal antibody nucleic acid hybridization nucleic acid probes nucleic acid sequence placenta polysomes postmortem prosencephalon radiotracer
中文摘要
胆碱乙酰转移酶(ChAT)的单克隆和多克隆抗体
将用于免疫分离编码ChAT的信使RNA
酵素 人类胎盘组织,已被证明是一个丰富的来源,
的ChAT,将被用作该努力的多聚核糖体的来源。 人类
组织(通过阿尔茨海默病患者的尸检获得,
控制谁死于其他原因)从基底前脑区域将是
用于分离mRNA,使得疾病特异性和区域特异性
可以创建互补DNA文库。 这些图书馆将
用于筛选cDNA克隆,其存在或不存在与
老年痴呆症 ChAT克隆和其他阿尔茨海默病相关的
克隆将被用于研究脑细胞的生长和发育,
通过原位杂交和RNA定量进行培养。
英文摘要
Monoclonal and polyclonal antibodies to choline acetyl transferase (ChAT)
will be used to immunoisolate messenger RNA which codes for the ChAT
enzyme. Human placental tissue, which has been shown to be a rich source
of ChAT, will be used as a source of polysomes for this effort. Human
tissue (obtained by autopsies of Alzheimer's disease patients as well as
controls who died of other causes) from the basal forebrain regions will be
utilized to isolated mRNA so that disease specific and region specific
libraries of complementary DNA can be created. These libraries will be
used to screen for cDNA clones whose presence or absence is associated with
Alzheimer's disease. The ChAT clones and the other Alzheimer related
clones will be used to study the growth and development of brain cells in
culture via in situ hybridization and RNA quantitation.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
Increased N-myc mRNA expression associated with dibutyryl cyclic AMP induced neuroblastoma differentiation.
与二丁酰环 AMP 诱导的神经母细胞瘤分化相关的 N-myc mRNA 表达增加。
DOI:
10.3109/01677068909107102
发表时间:
1989
期刊:
Journal of neurogenetics
影响因子:
1.9
作者:
[Berman,SA, Bursztajn,S, Kinnard,R, Cashman,N, Arnason,BG]
通讯作者:
Arnason,BG
MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
-
批准号:3078395
-
项目类别:
-
资助金额:$5.72万
-
财政年份:1985
-
负责人:STEPHEN A BERMAN
-
依托单位:
MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
-
批准号:3078396
-
项目类别:
-
资助金额:$5.72万
-
财政年份:1985
-
负责人:STEPHEN A BERMAN
-
依托单位:
MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
-
批准号:3078394
-
项目类别:
-
资助金额:$5.72万
-
财政年份:1985
-
负责人:STEPHEN A BERMAN
-
依托单位:
MOLECULAR BIOLOGY OF CHOLINERGIC NEURONS
-
批准号:3078397
-
项目类别:
-
资助金额:$5.72万
-
财政年份:1985
-
负责人:STEPHEN A BERMAN
-
依托单位:
海外基金