DEFINING MUCOSAL LYMPHOCYTE SUBPOPULATION

定义粘膜淋巴细胞亚群

• 批准号: 3080934
• 负责人: GARY J RUSSELL
• 金额: $ 8.2万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-15 至 1996-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3080934
• 关键词: T cell receptor; T lymphocyte; cell mediated lymphocytolysis test; cell population study; flow cytometry; gastrointestinal epithelium; human tissue; immunocytochemistry; immunofluorescence technique; inflammatory bowel diseases; intestinal mucosa; iodine; laboratory mouse; lymphocyte proliferation; monoclonal antibody; radiotracer; stable isotope; surface antigens; tissue /cell culture

The inflammatory bowel diseases (IBD), ulcerative colitis and Crohn's disease, are histologically characterized by an accumulation of lymphocytes and plasma cells in the lamina propria and foci of polymorphonuclear lymphocytes. An immune mechanism has long been suspected in the pathogenesis of IBD but, the etiology remains unknown. It is also not clear whether alterations in lymphocyte populations or their function mediate the mucosal injury or are epiphenomena of inflammation. Current research efforts investigating the functional properties of intestinal mucosal lymphocyte subpopulations, especially the intraepithelial lymphocytes (IEL), have been hampered by the inability to specifically identify IEL in cell suspension after isolation from intestinal specimens. The major goal of this project is to define possible phenotypic and functional differences in the mucosal lymphocyte subpopulations in IBD and normal intestine. This will be accomplished by using recently developed monoclonal antibodies that are primarily restricted to mucosal lymphocytes and IEL in particular. Monoclonal antibodies that recognize the T cell receptor gamma/delta will also be used to determine the intestinal distribution of these cells in IBD and their functional characteristics. A difference in the distribution or function of lymphocyte subpopulations in the lamina propria or epithelial compartments, may play a role in the pathogenesis of IBD.

炎症性肠病（IBD）、溃疡性结肠炎和克罗恩病 疾病，在组织学上的特征是淋巴细胞的积累 固有层和多形性腺瘤病灶中的浆细胞 淋巴细胞 长期以来，人们一直怀疑免疫机制 IBD的发病机制，但病因仍然不明。 也不 明确淋巴细胞群或其功能的改变 介导粘膜损伤或者是炎症的附带现象。 电流 研究肠道功能特性的研究工作 粘膜淋巴细胞亚群，尤其是上皮内淋巴细胞亚群， 淋巴细胞（IEL），由于不能特异性地 在从肠样本分离后鉴定细胞悬浮液中IEL。 该项目的主要目标是确定可能的表型和 IBD中粘膜淋巴细胞亚群的功能差异， 正常的肠道 这将通过使用最近开发的 主要限于粘膜淋巴细胞的单克隆抗体 尤其是IEL。 识别T细胞的单克隆抗体 受体γ/δ也将用于确定肠 这些细胞在IBD中的分布及其功能特征。 一 淋巴细胞亚群分布或功能的差异， 固有层或上皮隔室，可能在 IBD的发病机制