ROLE OF G-KINASE IN DIFFERENTIATION--USE OF HL-60 MUTANT

G-激酶在分化中的作用--HL-60 突变体的使用

基本信息

  • 批准号:
    3080021
  • 负责人:
  • 金额:
    $ 9.09万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1990
  • 资助国家:
    美国
  • 起止时间:
    1990-08-16 至 1995-07-31
  • 项目状态:
    已结题

项目摘要

The abnormal cells from patients with myelodysplastic syndromes and acute myelogenous leukemias fail to differentiate normally. A better understanding of the biochemical events regulating myeloid cell differentiation could potentially lead to more specific therapies of these disorders. The human promyelocytic cell line HL-60 provides the opportunity to study myeloid cell differentiation in vitro as it differentiates into granulocytes in response to several inducers, including dimethylsulfoxide (DMSO), and cAMP- and cGMP-elevating agents. Since cAMP and cGMP exert their effects almost exclusively through their respective protein kinases, it appears that differentiation of HL-60 cells can be induced by phosphorylation of key regulatory protein(s). The PI has recently isolated stable mutant HL-60 sublines which are resistant to the differentiating effects of elevated cGMP concentrations but differentiate normally in re- sponse to other inducing agents including DMSO and 8-Br-cAMP. Preliminary characterization of these mutants indicates a defect in phosphorylation of a cGMP-dependent protein kinase substrate (or substrates). The three major goals of this proposal are: (i) to identify the proteins phosphorylated in wild type HL-60 cells during cGMP-induced differentiation that are not phosphorylated in the mutant HL-60 cells; (ii) to purify and partially sequence one or several of these phosphoproteins; and (iii) to isolate a cDNA clone of one of the phosphoproteins. The proteins will be identified by 2-D PAGE/autoradiography and selected ones will be purified using a combination of radio-HPLC and PAGE/autoradiography. The partial amino acid sequence of the proteins will provide information about their physiological function and will allow synthesis of degenerative oligonucleotides and peptide specific antibodies. A cDNA clone and peptide-specific antibodies will allow study of the regulation of the phosphoprotein's synthesis during differentiation of HL-60 cells and of the molecular defect in the mutant HL-60 cells. The cloned gene will be transfected into the mutant cells to determine if it will correct their defect and the phosphoprotein will be microinjected into wild type HL-60 cells to determine if it is sufficient to induce differentiation. These studies will provide the PI with training in protein biochemistry and molecular biology and lay the foundation for future studies in patients with myelodysplastic syndromes and acute myelogenous leukemias.
骨髓增生异常综合征和急性骨髓增生异常综合征患者的异常细胞

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

RENATE B PILZ其他文献

RENATE B PILZ的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('RENATE B PILZ', 18)}}的其他基金

PKG Regulation of Sirtuin 1 as a Novel Treatment Strategy for Age-related Osteoporosis
Sirtuin 1 的 PKG 调节作为年龄相关性骨质疏松症的新型治疗策略
  • 批准号:
    10634657
  • 财政年份:
    2021
  • 资助金额:
    $ 9.09万
  • 项目类别:
PKG Regulation of Sirtuin 1 as a Novel Treatment Strategy for Age-related Osteoporosis
Sirtuin 1 的 PKG 调节作为年龄相关性骨质疏松症的新型治疗策略
  • 批准号:
    10296605
  • 财政年份:
    2021
  • 资助金额:
    $ 9.09万
  • 项目类别:
PKG Regulation of Sirtuin 1 as a Novel Treatment Strategy for Age-related Osteoporosis
Sirtuin 1 的 PKG 调节作为年龄相关性骨质疏松症的新型治疗策略
  • 批准号:
    10478942
  • 财政年份:
    2021
  • 资助金额:
    $ 9.09万
  • 项目类别:
Targeting defective NO/cGMP signaling as novel therapy for diabetic osteoporosis
针对缺陷的 NO/cGMP 信号作为糖尿病骨质疏松症的新疗法
  • 批准号:
    9899734
  • 财政年份:
    2016
  • 资助金额:
    $ 9.09万
  • 项目类别:
A novel treatment of aortic disease in Marfan Syndrome targeting oxidative stress and PKG dysregulation
针对氧化应激和 PKG 失调的马凡综合征主动脉疾病的新疗法
  • 批准号:
    10453951
  • 财政年份:
    2016
  • 资助金额:
    $ 9.09万
  • 项目类别:
Targeting defective NO/cGMP signaling as novel therapy for diabetic osteoporosis
针对缺陷的 NO/cGMP 信号作为糖尿病骨质疏松症的新疗法
  • 批准号:
    9459312
  • 财政年份:
    2016
  • 资助金额:
    $ 9.09万
  • 项目类别:
Targeting defective NO/cGMP signaling as novel therapy for diabetic osteoporosis
针对缺陷的 NO/cGMP 信号作为糖尿病骨质疏松症的新疗法
  • 批准号:
    9106282
  • 财政年份:
    2016
  • 资助金额:
    $ 9.09万
  • 项目类别:
A novel treatment of aortic disease in Marfan Syndrome targeting oxidative stress and PKG dysregulation
针对氧化应激和 PKG 失调的马凡综合征主动脉疾病的新疗法
  • 批准号:
    10588164
  • 财政年份:
    2016
  • 资助金额:
    $ 9.09万
  • 项目类别:
REGULATION OF GENE EXPRESSION BY NO/CGMP
NO/CGMP 对基因表达的调节
  • 批准号:
    6180686
  • 财政年份:
    1998
  • 资助金额:
    $ 9.09万
  • 项目类别:
REGULATION OF GENE EXPRESSION BY NO/CGMP
NO/CGMP 对基因表达的调节
  • 批准号:
    2630952
  • 财政年份:
    1998
  • 资助金额:
    $ 9.09万
  • 项目类别:

相似海外基金

Hedgehog signalling in T-cell differentiation and function
T 细胞分化和功能中的 Hedgehog 信号传导
  • 批准号:
    BB/Y003454/1
  • 财政年份:
    2024
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Research Grant
Comparative single-cell analysis of disease-derived stem cells to identify the cell fate defect on the cell differentiation trajectory
对疾病来源的干细胞进行比较单细胞分析,以确定细胞分化轨迹上的细胞命运缺陷
  • 批准号:
    23H02466
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The role of cell differentiation in colorectal cancer progression
细胞分化在结直肠癌进展中的作用
  • 批准号:
    23K06661
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
TOX-driven CD8 T cell differentiation and dysfunction in tumors
TOX驱动的肿瘤中CD8 T细胞分化和功能障碍
  • 批准号:
    10586679
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
Dissecting the role of hypoxia in T cell differentiation in cancer
剖析缺氧在癌症 T 细胞分化中的作用
  • 批准号:
    10578000
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
Mechanisms mediating human enteroendocrine cell differentiation and function
介导人肠内分泌细胞分化和功能的机制
  • 批准号:
    10739834
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
Elucidation of molecular mechanisms of immune cell differentiation of a novel Rab protein in hematopoietic stem cells
阐明造血干细胞中新型Rab蛋白免疫细胞分化的分子机制
  • 批准号:
    23K16122
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
New strategies in cell replacement therapies for diabetes: role of USP7 in iPSC and adult organoids beta cell differentiation
糖尿病细胞替代疗法的新策略:USP7 在 iPSC 和成体类器官 β 细胞分化中的作用
  • 批准号:
    MR/X01813X/1
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Research Grant
Role of alveolar fibroblasts in extracellular matrix organization and alveolar type 1 cell differentiation
肺泡成纤维细胞在细胞外基质组织和肺泡1型细胞分化中的作用
  • 批准号:
    10731854
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
Exhaustive Identification of Essential Genes for Human Taste Cell Differentiation ~Development of a Method for Inducing Differentiation of Taste Buds from ES/iPS Cells~
彻底鉴定人类味觉细胞分化必需基因~开发诱导ES/iPS细胞味蕾分化的方法~
  • 批准号:
    23K09214
  • 财政年份:
    2023
  • 资助金额:
    $ 9.09万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了