EFFECT OF OTOTOXICITY ON AUDITORY EVOKED POTENTIALS

拟议研究的重点是检查急性和慢性影响。 耳毒性制剂对耳蜗电生理活动的影响， 经无创耳蜗术(ECOG)检查的神经、脑干 和听性脑干反应(ABR)。其主要目的是 确定是否可以使用ECOG或ABR的变化来预测即将发生的情况 听力损失或在听力损失发生之前提供听力损失的早期识别 不可逆转。如果这些措施确实提供了这样的指标，那么其有效性 病人护理的质量可能会得到加强。实验将从以下方面开始 经鼓室无创脑电记录技术的研究进展 后续手术所需的龙猫耳膜(TM)和耳道 耳毒性的慢性动物研究。同步记录非侵入性 和侵入性测量总和电位(SP)的幅度、动作 然后，潜在(AP)幅度、AP延迟和AP阈值 比较一下。无创脑电地形图记录技术在这些地区的发展 动物将不再需要手术，特别是重复手术 ，在五月份由我们和其他人进行的动物研究。因此，需要 用于术后镇痛剂和抗生素以及无菌手术室 可以显著减少动物在慢性疾病中的痛苦 实验。然后，非侵入性记录技术在 动物和以前开发的无创记录技术 成年人的TM将被用来检查耳毒性的慢性影响 药物对SP波幅、AP波幅、SP/AP波幅比、AP的影响 延迟强度函数、AP输入输出函数、AP和ABR 听性脑干的阈值和适应与I-V间期 回应。在人类成年人中，行为测听阈值也将 被监视。在动物身上，将进行耳蜗学的组织学研究 在牺牲之后。在第三年，我们计划将动物研究扩大到 包括耳毒性和频率选择性单一单元研究 以单单元记录、ECOG记录或两者兼而有之。此外, 我们计划扩大无创耳蜗术的发展 对婴幼儿和新生儿的技术。婴幼儿和新生儿接受 然后可以包括耳毒性药物进行研究。在整个赠款过程中 在此期间，私家侦探将学习化学、药理学和 电子显微镜。同时，她将教书和督导 耳鼻咽喉科住院医师、医学生和药理学博士 学生在脑干听觉诱发电位、ABR和耳毒性方面。

The focus of the proposed study is to examine the acute and chronic effects of ototoxic preparations on the electrophysiologic activity of the cochlea, VIII nerve, and brainstem through noninvasive electrocochleography (ECOG) and the auditory brainstem response (ABR). The primary purpose is to determine if changes in the ECOG or ABR can be used to predict impending hearing loss or provide early identification of a hearing loss before it is irreversible. If these measures do provide such an indicant, the efficacy of patient care may be enhanced. Experiments will begin with the development of noninvasive ECOG recording techniques from the tympanic membrane (TM) and ear canal of chinchillas needed for the subsequent chronic animal study of ototoxicity. Simultaneously recorded noninvasive and invasive measures of the summating potential (SP) amplitude, action potential (AP) amplitude, AP latency, and AP threshold will then be compared. Development of noninvasive ECOG recording techniques in these animals will eliminate the need for surgery, particularly repeated surgery , in may animal studies by ourselves and others. Consequently, the need for postoperative analgesics and antibiotics and sterile operating rooms could be markedly curtailed as well as possible animal suffering in chronic experiments. Then the noninvasive recording techniques developed in animals and previously developed noninvasive recording techniques from the TM in adult humans will be used to examine the chronic effects of ototoxic agents on SP amplitude, AP amplitude, the SP/AP amplitude ratio, AP latency-intensity functions, AP input-output functions, AP and ABR threshold and adaptation and the I-V interval of the auditory brainstem response. In the human adults, behavioral audiometric thresholds will also be monitored. In the animals, histology of the cochlea will be performed after sacrifice. In the third year, we plan to expand animal studies to include single unit studies of ototoxicity, and frequency selectivity either in single unit recordings, ECOG recordings, or both. In addition, we plan to expand the development of noninvasive electrocochleographic techniques to infants and neonates. Infants and neonates receiving ototoxic agents could then be included for study. Throughout the grant period the P.I. will take formal coursework in chemistry, pharmacology, and electron microscopy. Simultaneously, she will teach and supervise otolaryngology residents, medical students, and pharmacology doctoral students in the area of ECOG, ABR, and ototoxicity.