CONNECTIONS OF SUPERFICIAL DORSAL HORN NEURONS

表层背角神经元的连接

• 批准号: 3078209
• 负责人: DAVID DUBUISSON
• 金额: $ 4.26万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1989-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3078209
• 关键词: analgesia; antidromic impulse; axon; cats; dorsal column; histochemistry /cytochemistry; interneurons; laboratory rat; microelectrodes; neurochemistry; neuropeptide receptor; pain; single cell analysis; spinal cord mapping

This research will examine the connections, functional properties and chemical content of neurons in the dorsal horn of the spinal cord in decerebrate cats and rats. Further understanding of the role of these cells could lead to better means of treating severe pain in humans. Four related projects are proposed: (1) Superficial dorsal horn neurons of laminae 1-2 and deeper neurons of laminae 3-6 which show responses to stimulation of descending pathways relevant to opiate and 'stimulation-produced' analgesia will be characterized by intracellular dye-labelling and by immunohistochemical staining for peptide or enzyme content. (2) Axon trajectories of neurons in laminae 1-2 will be traced by extracellular and intracellular single unit recording and systematic detailed antidromic mapping using stimulation of varying intensities delivered through low-impedance microelectrodes. Representative units will be marked by intracellular staining and examined immunohistochemically. (3) Activity in pairs of dorsal horn neurons, one in laminae 1-3 and the second deeper in laminae 3-6, will be correlated using simultaneous single unit recordings, spike-triggered averaging, and computation of poststimulus time histograms during repetitive stimulation of descending and afferent systems. If consistent patterns of correlation are found, representative units -- one or both of pair -- will be identified by intracellular staining and by immunohistochemical techniques. (4) Receptive fields and activity of individual neurons in the superficial dorsal horn will be examined before, during and after induction of general anesthesis with pentobarbital or alpha-chloralose. Individual units will be followed by prolonged extracellular single unit recordings with repeated mapping of peripheral receptive fields. Representative units will be penetrated and identified by intracellular dye marking.

这项研究将检查连接、功能特性和 脊髓背角神经元的化学成分 使猫和老鼠去大脑。 进一步了解这些的作用 细胞可能会带来更好的方法来治疗人类的严重疼痛。 四 提出相关项目：（1）浅层背角神经元 层 1-2 和层 3-6 的更深神经元表现出对 刺激与阿片相关的下行通路 “刺激产生”镇痛的特点是细胞内 染料标记和肽或酶的免疫组织化学染色 内容。 (2) 1-2 层神经元的轴突轨迹将通过以下方式追踪 细胞外和细胞内单个单元记录和系统 使用不同强度的刺激进行详细的逆向绘图 通过低阻抗微电极传递。 代表单位将 通过细胞内染色进行标记并进行免疫组织化学检查。 (3) 成对背角神经元的活动，其中一个位于第 1-3 层， 第二个更深的层 3-6，将使用同时单个 单位记录、尖峰触发平均和刺激后计算 下行和传入重复刺激期间的时间直方图 系统。 如果发现一致的相关模式，则具有代表性 单位——一对或两者——将由细胞内识别 染色和免疫组织化学技术。 (4) 感受野和 浅表背角单个神经元的活动将 在全身麻醉诱导之前、期间和之后进行检查 戊巴比妥或α-氯醛糖。 个别单位将紧随其后 长时间的细胞外单个单元记录与重复映射 外周感受野。 代表性单位将被渗透 通过细胞内染料标记来识别。