BIOGENESIS OF SURFACE MEMBRANE OF SCHISTOSOMA MANSONI

医师科学家奖的长期目标 应用是发展一项独立的生物医学研究 在细胞生物学方面的职业生涯，并将这一子专业应用于研究 血吸虫病。第一阶段将由两个主要部门组成。 首先，我们将介绍细胞生物学的基础知识 利用研究生院的课程、研讨会、期刊俱乐部和 非正式讨论。一个委员会将监督 候选人。第二，一个关于人造卫星生物学的研究项目 曼氏血吸虫的表面成熟将在 布朗大学的肯尼斯·米勒博士和G·理查德·奥兹博士。 这些研究将调查表面成熟过程中的 感染性尾虫向血吸虫幼虫的转化 寄生虫必须从自由生活适应到 寄生的存在。第一，第二信使在 表面成熟度的启动将被研究。接下来，因为 微管抑制剂阻止表面成熟并减少 血吸虫幼虫成熟成成虫、微管的能力 将进行深入研究。最后，血吸虫表面抗原 将通过免疫细胞化学重新定位在尾蚴内。 尾蚴内抗原的检测可能表明疫苗 抗血吸虫病可从尾虫中制备 舞台。 在完成两年的学习后，应聘者将发展为 从事独立研究的基本技能和教育 第二阶段。这项研究将是对 血吸虫表面膜。了解细胞 膜成熟过程中的生物学机制 尾虫-血吸虫的转化可能会导致 阻断血吸虫病传播的新策略。

The long term objective of the Physician Scientist Award application is to develop an independent biomedical research career in cellular biology and apply this subspecialty to the study of schistosomiasis. Phase I will consist of 2 major divisions. First, a fundamental background in cell biology will be developed utilizing graduate school courses, seminars, journal clubs and informal discussions. A committee will oversee the progress of the candidate. Second, a research project on the biology of surface maturation of Schistosoma mansoni will be initiated under Drs. Kenneth Miller and G. Richard Olds of Brown University. These studies will investigate surface maturation during the transformation of infective cercariae to larval schistosomula, a critical period when the parasite must adapt from a free-living to a parasitic existence. First, the role of second messengers in initiation of surface maturation will be investigated. Next, since microtubule inhibitors prevent surface maturation and reduce the ability of schistosomula to mature into adult worms, microtubules will be studied in depth. Finally, schistosomula surface antigens will belocalized within cercariae using immunocytochemistry. Detection of antigens within cercariae may suggest that vaccines against schistosomiasis could be prepared from the cercarial stage. At the completion of 2 years the candidate will have developed basic skills and education to pursue independent research during Phase II. This research will be a continuation of the study of schistosomes surface membranes. Understanding the cell biological mechanisms of membrane maturation during the cercarial-schistosomula transformation may allow development of new strategies to interrupt transmission of schistosomiasis.