BIOGENESIS OF SURFACE MEMBRANE OF SCHISTOSOMA MANSONI

医生科学家奖的长期目标 申请是发展一个独立的生物医学研究 在细胞生物学的职业生涯，并应用这个亚专业的研究 血吸虫病 第一阶段将包括两个主要部门。 首先，在细胞生物学的基本背景将得到发展 利用研究生院的课程，研讨会，期刊俱乐部和 非正式讨论。 一个委员会将监督 候选人。 第二，一个关于生物学的研究项目， 曼氏血吸虫的表面成熟将在 Drs. Kenneth米勒和G.布朗大学的理查德奥尔兹。 这些研究将调查在 感染性尾蚴转化为幼虫的囊蚴，a 关键时期，寄生虫必须适应从自由生活到 寄生的存在 第一，第二信使的作用 将研究表面熟化的起始。 接下来，由于 微管抑制剂阻止表面成熟并减少细胞的增殖。 童虫成熟为蠕虫、微管的能力 将深入研究。 最后，童虫表面抗原 用免疫细胞化学将其定位在尾蚴内。 尾蚴内抗原的检测可能表明疫苗 抗血吸虫病的制剂 阶段 2年后，候选人将发展 基本技能和教育，以追求独立研究期间 第二阶段。 本研究将是 囊体表面膜。 理解细胞 膜成熟的生物学机制 尾蚴-囊胚转化可能允许发育成 阻断血吸虫病传播的新策略。

The long term objective of the Physician Scientist Award application is to develop an independent biomedical research career in cellular biology and apply this subspecialty to the study of schistosomiasis. Phase I will consist of 2 major divisions. First, a fundamental background in cell biology will be developed utilizing graduate school courses, seminars, journal clubs and informal discussions. A committee will oversee the progress of the candidate. Second, a research project on the biology of surface maturation of Schistosoma mansoni will be initiated under Drs. Kenneth Miller and G. Richard Olds of Brown University. These studies will investigate surface maturation during the transformation of infective cercariae to larval schistosomula, a critical period when the parasite must adapt from a free-living to a parasitic existence. First, the role of second messengers in initiation of surface maturation will be investigated. Next, since microtubule inhibitors prevent surface maturation and reduce the ability of schistosomula to mature into adult worms, microtubules will be studied in depth. Finally, schistosomula surface antigens will belocalized within cercariae using immunocytochemistry. Detection of antigens within cercariae may suggest that vaccines against schistosomiasis could be prepared from the cercarial stage. At the completion of 2 years the candidate will have developed basic skills and education to pursue independent research during Phase II. This research will be a continuation of the study of schistosomes surface membranes. Understanding the cell biological mechanisms of membrane maturation during the cercarial-schistosomula transformation may allow development of new strategies to interrupt transmission of schistosomiasis.