PATHOBIOLOGY OF PARTHENOGENESIS--MOLECULAR CONTROLS

孤雌生殖是一种生物现象，通过这种现象，细胞分裂和 在激活未受精的配子后发生分化。 这是一种新的病理事件，减数分裂细胞异常进入。 有丝分裂。这一过程类似于细菌的肿瘤转化。 卵巢畸胎瘤是人类最常见的生殖细胞肿瘤。 单倍体动物为研究细胞的遗传调控提供了一个独特的模型 一旦启动，它们在时间和时间上不同于正常细胞 细胞和有丝分裂的同步化。虽然有几个关键 已经描述了细胞周期的调节，完整的途径和 体内相互作用的全光谱还有待定义。此外， 单元体之所以特别有趣，是因为它们集成在一个系统中 发育和肿瘤共同的功能成分。 单倍体可以在体外产生和操纵，从而促进 描述性和功能性研究。中描述的实验的目的 这项建议是为了定义涉及到的候选基因的表达 细胞周期基因、细胞癌基因、生长因子等孤雌生殖 基因和分化基因。到目前为止，这一地区还没有人研究过。 单倍体将在体外产生，用于随后的PCR分析 C DNA的扩增。候选基因的表达将与 单性生殖发育的表型。假设是后遗症- 激活孤雌生殖事件受差异调节控制 与正常胚胎相比，候选基因的数量。

Parthenogenesis is the biologic phenomenon whereby cell division and differentiation occurs following activation of an unfertilized gamete. This is a novel pathologic event where meiotic cells aberrantly enter mitosis. This process is analagous to neoplastic transformation of germ cells into ovarian teratomas, the most common human germ cell tumor. Parthenotes offer a unique model to study the genetic regulation of cell division; once initiated, they differ from normal cells in timing and synchronization of cyto- and karyo- kinesis. Although several key regulators of the cell cycle have been described, the complete pathways and full spectrum of in vivo interactions have yet to be defined. Furthermore, parthenotes are of particular interest because they integrate in one system the functional elements common to both development and neoplasia. Parthenotes may be generated and manipulated in vitro, facilitating descriptive and functional studies. The aims of experiments described in this proposal are to define the expression of candidate genes involved in parthenogenesis such as cell cycle genes, cellular oncogenes, growth factor genes, and differentiation genes. This area has been unstudied to date. Parthenotes will be generated in vitro for subsequent mRNA analysis by PCR amplification of cDNA. Expression of candidate genes will be correlated to the phenotype of parthenote development. The hypothesis is that post- activation parthenogenic events are controlled by differential regulation of candidate genes as compared to normal embryos.