PATHOBIOLOGY OF PARTHENOGENESIS--MOLECULAR CONTROLS

单性生殖的病理学--分子控制

• 批准号: 3085913
• 负责人: DRUCILLA J ROBERTS
• 金额: $ 7.43万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3085913
• 关键词: cell growth regulation; cytogenetics; disease /disorder model; embryogenesis; gene expression; genetic transcription; hematopoietic stem cells; in situ hybridization; laboratory mouse; messenger RNA; molecular cloning; molecular oncology; neoplasm /cancer genetics; neoplastic transformation; oncogenes; ovary neoplasms; parthenogenesis; synchronous cell division; teratoma

Parthenogenesis is the biologic phenomenon whereby cell division and differentiation occurs following activation of an unfertilized gamete. This is a novel pathologic event where meiotic cells aberrantly enter mitosis. This process is analagous to neoplastic transformation of germ cells into ovarian teratomas, the most common human germ cell tumor. Parthenotes offer a unique model to study the genetic regulation of cell division; once initiated, they differ from normal cells in timing and synchronization of cyto- and karyo- kinesis. Although several key regulators of the cell cycle have been described, the complete pathways and full spectrum of in vivo interactions have yet to be defined. Furthermore, parthenotes are of particular interest because they integrate in one system the functional elements common to both development and neoplasia. Parthenotes may be generated and manipulated in vitro, facilitating descriptive and functional studies. The aims of experiments described in this proposal are to define the expression of candidate genes involved in parthenogenesis such as cell cycle genes, cellular oncogenes, growth factor genes, and differentiation genes. This area has been unstudied to date. Parthenotes will be generated in vitro for subsequent mRNA analysis by PCR amplification of cDNA. Expression of candidate genes will be correlated to the phenotype of parthenote development. The hypothesis is that post- activation parthenogenic events are controlled by differential regulation of candidate genes as compared to normal embryos.

单性生殖是一种生物学现象， 分化发生在未受精配子的激活之后。 这是一个新的病理事件，减数分裂细胞异常进入 分裂。 这一过程类似于细菌的肿瘤转化 卵巢畸胎瘤是最常见的人类生殖细胞肿瘤。 单性生殖为研究细胞遗传调控提供了一个独特的模型 分裂;一旦启动，它们在时间上不同于正常细胞， 细胞和核分裂同步化。 虽然几个关键 已经描述了细胞周期的调节剂，完整的途径和 体内相互作用的全谱尚待确定。 此外，委员会认为， 单性生殖是特别感兴趣的，因为它们整合在一个系统中 发育和瘤形成的共同功能要素。 可以在体外产生和操作单性生殖体，促进 描述性和功能性研究。 中描述的实验的目的 这项建议是为了确定参与的候选基因的表达， 孤雌生殖，如细胞周期基因、细胞癌基因、生长因子 基因和分化基因。 这一领域迄今尚未研究。 将在体外产生单性生殖细胞，用于随后通过PCR进行mRNA分析 cDNA的扩增。 候选基因的表达将与 单性生殖发育的表型。 假设是后- 激活孤雌生殖事件受差异调节控制 与正常胚胎相比，