CELLULAR TROPISM OF HIV

HIV的细胞趋向性

• 批准号: 3085305
• 负责人: ROGER J POMERANTZ
• 金额: $ 8.94万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-10-01 至 1994-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3085305
• 关键词: DNA footprinting; T lymphocyte; genetic transcription; glycoproteins; growth factor; human immunodeficiency virus; macrophage; molecular cloning; monocyte; nucleic acid sequence; restriction mapping; transfection; virus envelope; virus protein; virus replication

This Physician/Scientist Award is designed to provide a rigorous and thorough scientific training in preparation for a career in academic medicine with intensive basic science research commitment. It combines the superb academic atmosphere at the Whitehead Institute, Massachusetts Institute of Technology, the Harvard Medical School and the Massachusetts General Hospital (MGH). The Phase One sponsor, Dr. David Baltimore, has an impressive enthusiasm for the nurture and training of young physician/ scientists. Phase One will involve an intensive research experience at the Whitehead Institute. At the same time, graduate level course work in molecular genetics, molecular virology and immunology will be conducted at Harvard, M.I.T. and the Marine Biological Laboratory at Woods Hole. In Dr. David Baltimore's laboratory, the research will focus on the understanding of Human immunodeficiency Virus (HIV) replication in the monocyte/macrophage. The extremely complex nature of HIV replication will be investigated using gel retardation assays and DNA protection assays to study cell-specific factors involved in directing the transcription of HIV proteins. An important cell-derived factor, NF Kappa B, which has been shown to be involved in HIV replication in T cell lines, will be studied in the monocyte/macrophage. We will also investigate whether there are genetic differences in a cell tropic versus monocyte/macrophage tropic HIV strains. Restriction enzyme analysis, cloning and nucleotide sequencing of specific HIV isolates with differing cell tropism will be performed to examine whether there exist specific genetic differences in HIV strains within the long terminal repeat and/or the envelope glycoprotein region. A rigorous understanding of HIV production in the monocyte/ macrophage will be key in understanding a variety of the pathogenetic mechanisms of this virus. During this time, the candidate will become familiar with a number of important and generalizable molecular virologic techniques.

这个医生/科学家奖旨在提供一个严格的 和全面的科学训练，为从事 学术医学与密集的基础科学研究的承诺。 它结合了怀特黑德一流的学术氛围， 学院、马萨诸塞州理工学院、哈佛 医学院和马萨诸塞州总医院（MGH）。 的 第一阶段的赞助商，大卫博士巴尔的摩，有一个令人印象深刻的 培养青年医师的热情 科学家 第一阶段将涉及密集的研究经验， 怀特黑德研究所。 同时，研究生水平的课程工作 在分子遗传学、分子病毒学和免疫学方面， 在麻省理工学院的哈佛大学进行。海洋生物实验室 在伍兹霍尔。 在巴尔的摩的大卫博士的实验室里， 将侧重于了解人类免疫缺陷病毒 (HIV)在单核细胞/巨噬细胞中复制。 极 HIV复制的复杂性将使用凝胶 阻滞试验和DNA保护试验，以研究细胞特异性 参与指导HIV蛋白质转录的因子。 一种重要的细胞衍生因子，NF κ B B，已被证明 参与HIV在T细胞系中的复制， 在单核细胞/巨噬细胞中。 我们还将调查是否有 是嗜细胞性与单核细胞/巨噬细胞的遗传差异 嗜性HIV毒株。 限制性内切酶分析、克隆和 不同细胞的特定HIV分离株的核苷酸测序 进行趋向性检查是否存在特异性 HIV病毒株在长末端重复序列内的遗传差异 和/或包膜糖蛋白区。 一个严格的理解 单核细胞/巨噬细胞中HIV的产生将是 了解这种疾病的各种发病机制， 病毒 在此期间，候选人将熟悉 一些重要的和可推广的分子病毒学 技术.