THE CELLULAR & MYONUCLEAR PHENOTYPE OF MYOSIN

细胞

• 批准号: 3084100
• 负责人: LINDA A SPECHT
• 金额: $ 8.75万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3084100
• 关键词: cell nucleus; cell type; gene expression; genetic regulation; histochemistry /cytochemistry; human tissue; immunochemistry; muscle cells; myogenesis; myosins; nucleic acid probes; sarcomeres; tissue mosaicism

The overall goal of this project is to characterize the cellular expression of the human sarcomeric myosin heavy chain (MHC) gene family and begin to understand the relationship between diversity of cellular phenotype and MHC gene expression at the level of the individual myonucleus (myonucleus phenotype). The cellular MHC phenotype of normal differentiated, developing and diseased human muscle in vivo will be established by immunocytochemical detection of the MHC isoform protein; the myonuclear phenotype will in turn be determined in vitro the used of available cDNA probes for MHC isoform genes and the technique of in situ hybridization. Characterization of the myonuclear phenotype will test the hypothesis that myofibers are mosaic with respect to myonuclear MHC phenotype. Finally, the ontogeny of hypothesized myonuclear diversity will be studied further by exploiting the chick-quail chimera paradigm to create experimental mosaic myotubes in vitro. Such a model will permit more extensive study of myogenesis and gene regulation.

该项目的总体目标是表征细胞 人肌节肌球蛋白重链（MHC）基因的表达 家庭和开始了解多样性之间的关系 细胞表型和MHC基因表达的水平， 单个肌核（肌核表型）。 细胞MHC 正常分化、发育和患病人的表型 通过免疫细胞化学检测建立体内肌肉 MHC同种型蛋白质; mycoplasma表型将依次 在体外确定可用的MHC cDNA探针的使用 同种型基因和原位杂交技术。 肌球蛋白表型的表征将测试 关于肌纤维与肌球蛋白MHC嵌合假说 表型 最后，假设的肌细胞的个体发育 通过对鸡鹌鹑的利用，进一步研究其多样性 嵌合体范例以在体外产生实验性嵌合肌管。 这样的模型将允许更广泛地研究肌发生， 基因调控