CELL AND MOLECULAR BIOLOGY OF NA+ AND K+ ATPASE

NA 和 K ATP酶的细胞和分子生物学

• 批准号: 3084098
• 负责人: LINDA A SPECHT
• 金额: $ 7.43万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-01-01 至 1992-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3084098
• 关键词: cell nucleus; cell type; gene expression; genetic regulation; histochemistry /cytochemistry; human tissue; immunochemistry; muscle cells; myogenesis; myosins; nucleic acid probes; sarcomeres; tissue mosaicism

The overall goal of this project is to characterize the cellular expression of the human sarcomeric myosin heavy chain (MHC) gene family and begin to understand the relationship between diversity of cellular phenotype and MHC gene expression at the level of the individual myonucleus (myonucleus phenotype). The cellular MHC phenotype of normal differentiated, developing and diseased human muscle in vivo will be established by immunocytochemical detection of the MHC isoform protein; the myonuclear phenotype will in turn be determined in vitro the used of available cDNA probes for MHC isoform genes and the technique of in situ hybridization. Characterization of the myonuclear phenotype will test the hypothesis that myofibers are mosaic with respect to myonuclear MHC phenotype. Finally, the ontogeny of hypothesized myonuclear diversity will be studied further by exploiting the chick-quail chimera paradigm to create experimental mosaic myotubes in vitro. Such a model will permit more extensive study of myogenesis and gene regulation.

这个项目的总体目标是描述细胞的特征 人肌球蛋白重链基因的表达 并开始了解多样性之间的关系 在细胞表型和MHC基因表达的水平上 个体肌核(肌核表型)。细胞MHC 正常分化、发育和疾病人群的表型 通过免疫细胞化学检测建立活体肌肉 MHC异构体蛋白；肌核表型将依次 可用于MHC的现有基因探针的体外检测 异构体基因和原位杂交技术。 肌核表型的特征将测试 肌纤维相对于肌核MHC是镶嵌的假说 表型。最后，假想肌核的个体发育 多样性将通过利用雏鸟来进一步研究。 嵌合体范例在体外创造实验性的马赛克肌管。 这样的模型将允许更广泛地研究肌肉发生和 基因调控。