X-RAY MICROANALYTICAL STUDY OF FLUORIDE DISTRIBUTION

龋病研究中的一个主要主题是摄取和 氟化物等元素在牙齿增多症中的分布 防龋性的。在这项工作中，使用了玻璃离子粘固剂 哪种结合到牙釉质和牙本质上并释放氟化物 巨大的潜力。已有研究试图确定 牙釉质、牙本质和牙骨质氟的摄取 采用公认的酸法制备的其他氟化物制剂 蚀刻活组织检查、电测和层析程序。 活组织检查技术在灵敏度上是有限的，而且 直接适用于牙本质研究，而色谱学 这一过程非常耗时，而且会破坏样品。都不是 方法可以显示除to以外的元素的实际分布 指定选定区域的离散值。 这项提议的目标是使定性和 元素含量及分布的定量研究 直接、可靠和可重复的矿化牙科组织 使用电子探针x射线能谱的方法 (微量分析)，并使用V类玻璃离子水门汀 以恢复剂为实验模型。此方法计算 超新星X射线的能量和波长特性 扫描电子显微镜过程中的样品。因为这些 发射X射线的元素的特性是独一无二的， 它们提供了定性和定量的基础 以灵敏和准确的方式测定这些元素。 由于x光照射，可以重复测量。 微量分析是非破坏性的。这使得这是一个很好的方法 为其在龋齿研究中的使用制定标准。 与其他方法不同，x射线分析可以应用于以下任何领域 牙釉质、牙骨质或牙本质以及凹槽、凹槽和 龋齿地区，从而使这成为一种重要的调查方法 在根面龋齿研究方面。这项研究将使用能量弥散和 波长色散X射线微区分析产生新的 对产品质量和数量特征的了解 矿化牙体组织对氟和氟的摄取和分布 玻璃离子粘固剂中的其他元素。X-射线技术 强度测绘和计算机图像分析将产生新的 关于元素实际分布的数据。这些研究将 提供有关内容的重要新信息和 氟化物等元素在龋病研究中的分布 流行和预防。

A major topic in caries research is the influence of the uptake and distribution of fluoride and other elements in making teeth more caries resistant. In this effort, the use of glass ionomer cements which bond to enamel and dentin and release fluoride has significant potential. Studies have attempted to determine the enamel, dentin, and cementum fluoride uptake from these and other fluoride preparations by the accepted methodologies of acid etch biopsy and electrometric and chromatographic procedures. The biopsy techniques are limited in sensitivity and are not directly applicable to dentin studies, and the chromatographic procedure is time consuming and destroys the sample. Neither method can show actual distribution of elements of except to state discrete values for selected areas. The objective of this proposal is to make qualitative and quantitative studies of the content and distribution of elements in mineralized dental tissues in a direct, reliable, and repeatable manner by using Electron Probe x-Ray Spectroscopy (microanalysis), and using Class V glass ionomer cement restoratives as the experimental model. This method evaluates the energy and wavelength characteristics of x-ray emitted by a specimen during scanning electron microscopy. Since these characteristics are unique of the elements emitting the x-rays, they provide the basis for qualitative and quantitative determinations of those elements in a sensitive and accurate way. Repeated measurements can be performed since x-ray microanalysis is non-destructive. This makes this a good method for developing standard criteria for its use in caries research. Unlike other methods, x-ray analysis can be applied to any area of enamel, cementum, or dentin as well as to pits, grooves and carious areas, thus making this an important investigative method in root caries research. This study will use energy dispersive and wavelength dispersive x-ray microanalysis to produce new knowledge of the qualitative and quantitative characteristics of mineralized dental tissue uptake and distribution of fluoride and other elements from glass ionomer cements. Techniques for x-ray intensity mapping and computer image analysis will yield new data on the actual distribution of elements. These studies will provide significant new information on the content and distribution of fluoride and other elements in the study of caries prevalence and prevention.