X-RAY MICROANALYTICAL STUDY OF FLUORIDE DISTRIBUTION

龋齿研究中的一个主要课题是吸收和吸收的影响。 氟和其他元素的分布，使牙齿更 抗龋齿。 在这项工作中，使用玻璃离子水门汀， 它与牙釉质和牙本质结合并释放氟化物， 巨大的潜力。 研究试图确定 牙釉质、牙本质和牙骨质从这些吸收氟化物， 用公认的酸法制备其他氟化物 蚀刻活组织检查和电测量以及色谱法。 活检技术的灵敏度有限， 直接适用于牙本质研究， 该过程是耗时的并且破坏样品。 既不 方法可以显示元素的实际分布， 为选定区域指定离散值。 本建议的目的是使质量和 元素含量和分布的定量研究 矿化牙组织，直接，可靠，可重复 电子探针X射线光谱法 （微量分析），并使用V类玻璃离子水门汀 作为实验模型。 该方法评估 X射线的能量和波长特性 扫描电子显微镜下的样品。 由于这些 发射X射线的元件的特性是独特的， 它们提供了定性和定量的基础 以敏感和准确的方式测定这些元素。 可以重复测量，因为X射线 微量分析是非破坏性的。 这使得这是一个好方法 用于制定其在龋齿研究中使用的标准。 与其他方法不同，X射线分析可以应用于任何领域， 牙釉质、牙骨质或牙本质以及小坑、凹槽和 因此，这是一种重要的调查方法 在牙根龋研究中。 这项研究将使用能量分散和 波长色散X射线显微分析，以产生新的 知识的定性和定量特征 矿化的牙齿组织吸收和分布的氟化物和 玻璃离子水门汀的其他元素。 X射线技术 强度映射和计算机图像分析将产生新的 元素的实际分布数据。 这些研究将 提供有关内容的重要新信息， 氟等元素在龋病研究中的分布 流行和预防。

A major topic in caries research is the influence of the uptake and distribution of fluoride and other elements in making teeth more caries resistant. In this effort, the use of glass ionomer cements which bond to enamel and dentin and release fluoride has significant potential. Studies have attempted to determine the enamel, dentin, and cementum fluoride uptake from these and other fluoride preparations by the accepted methodologies of acid etch biopsy and electrometric and chromatographic procedures. The biopsy techniques are limited in sensitivity and are not directly applicable to dentin studies, and the chromatographic procedure is time consuming and destroys the sample. Neither method can show actual distribution of elements of except to state discrete values for selected areas. The objective of this proposal is to make qualitative and quantitative studies of the content and distribution of elements in mineralized dental tissues in a direct, reliable, and repeatable manner by using Electron Probe x-Ray Spectroscopy (microanalysis), and using Class V glass ionomer cement restoratives as the experimental model. This method evaluates the energy and wavelength characteristics of x-ray emitted by a specimen during scanning electron microscopy. Since these characteristics are unique of the elements emitting the x-rays, they provide the basis for qualitative and quantitative determinations of those elements in a sensitive and accurate way. Repeated measurements can be performed since x-ray microanalysis is non-destructive. This makes this a good method for developing standard criteria for its use in caries research. Unlike other methods, x-ray analysis can be applied to any area of enamel, cementum, or dentin as well as to pits, grooves and carious areas, thus making this an important investigative method in root caries research. This study will use energy dispersive and wavelength dispersive x-ray microanalysis to produce new knowledge of the qualitative and quantitative characteristics of mineralized dental tissue uptake and distribution of fluoride and other elements from glass ionomer cements. Techniques for x-ray intensity mapping and computer image analysis will yield new data on the actual distribution of elements. These studies will provide significant new information on the content and distribution of fluoride and other elements in the study of caries prevalence and prevention.