ROLE OF THROMPOSPONDIN IN ODONTOGENESIS

凝血反应蛋白在成牙过程中的作用

• 批准号: 3086203
• 负责人: RONALD W KATZ
• 金额: $ 9.02万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-30 至 1995-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3086203
• 关键词: cell adhesion molecules; complementary DNA; dental development; epithelium; extracellular matrix; gene expression; genetically modified animals; hormone regulation /control mechanism; immunocytochemistry; in situ hybridization; laboratory mouse; laboratory rat; mesenchyme; molecular biology; molecular cloning; physical chemical interaction; restriction fragment length polymorphism; site directed mutagenesis; thrombospondins

The purpose of this award is to provide the applicant with the opportunity to obtain the skills necessary to become a fully independent biomedical investigator with expertise in the application of molecular biological techniques to questions of the pathology of epithelial-mesenchymal interactions. With these skills, the applicant will apply his training towards research in craniofacial development and oral pathology while developing a career in academic dentistry. The initial phase of the award will be spent in didactic study within the interdisciplinary program in Cellular and Molecular Biology at the University of Michigan and in laboratory rotations learning different techniques of molecular biology (RFLP analysis, transgeneic mouse procedures, cloning technology, site directed mutagenesis, cDNA expression in mammalian cells, in-situ hybridization, immunolocalization of target proteins in embryologic tissues). The final phase will consist of application of these research tools to investigate the role of an extracellular, cell adhesion protein (thrombospondin) in odontogenesis. The aim of this research is to integrate the role of thrombospondin to the rapidly expanding knowledge available on cell adhesion proteins, other extracellular matrix proteins, and cellular transmembrane receptors in the epithelial-mesenchymal interactions occurring in tooth formation. Since tooth formation is a model to study the way genes regulate the spatial and temporal formation of organs dependent on the interaction of different embryonic cell lines, information obtained from this model should be applicable to the development (normal and aberrant) of other organ systems.

该奖项的目的是为申请人提供机会 获得成为完全独立的生物医学人员所需的技能 具有分子生物学应用专业知识的研究员 上皮间质病理学问题的技术 互动。有了这些技能，申请人将能够应用他所接受的培训 致力于颅面发育和口腔病理学的研究，同时 发展学术牙科事业。奖项初始阶段 将用于跨学科计划内的教学研究 密歇根大学的细胞和分子生物学 实验室轮转学习不同的分子生物学技术 （RFLP 分析、转基因小鼠程序、克隆技术、位点 定向诱变，哺乳动物细胞中的 cDNA 表达，原位 胚胎学中靶蛋白的杂交、免疫定位 组织）。最后阶段将包括这些研究的应用 研究细胞外细胞粘附蛋白作用的工具 （血小板反应蛋白）在牙发生过程中。这项研究的目的是整合 血小板反应蛋白对快速扩展的知识的作用 细胞粘附蛋白、其他细胞外基质蛋白和细胞 上皮间质相互作用中的跨膜受体 发生在牙齿形成过程中。由于牙齿形成是研究牙齿形成的模型 基因调节器官依赖的空间和时间形成的方式 关于不同胚胎细胞系的相互作用，获得的信息 从这个模型应该适用于开发（正常和 其他器官系统的异常）。