MINORITY SCHOOL FACULTY DEVELOPMENT AWARD

少数族裔学校教师发展奖

• 批准号: 3088293
• 负责人: William L Stone
• 金额: $ 5.24万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088293
• 关键词: antioxidants; blood lipoprotein biosynthesis; blood lipoprotein metabolism; dietary lipid; dietary trace element; human tissue; hyperlipoproteinemia; lipid peroxides; liver metabolism; low density lipoprotein; malnutrition; nutrition related tag; peroxidation; selenium; very low density lipoprotein; vitamin E deficiency

The role of antioxidant nutrients in lipid metabolism has not been well defined. We have found that rats fed a diet deficient in antioxidant nutrients (selenium and vitamin E) develop elevated levels of low density lipoprotein-cholesterol (LDL-cho). The development of elevated LDL-cho in spontaneously hypertensive (SHR) rats fed a 1% cholesterol diet deficient in selenium (Se) and vitamin E is particularly accelerated compared with other rat strains. Our primary research objective is to characterize and determine the metabolic and biochemical mechanism(s) responsible for the alterations in rat plasma lipoprotein levels resulting from dietary deficiencies of antioxidant nutrients. Cholesterol free diets, as well as diets containing 1% cholesterol will be utilized. The animals to be studied are inbred SHR rats, and control normotensive (WKY) rats. We will study lipoprotein composition, LDL catabolism, and VLDL synthesis in antioxidant deficient and sufficient rats. The plasma clearance and tissue uptake of LDL will be determined in intact animals. LDL uptake and VLDL synthesis will be studied in a liver perfusion system. A second long term goal is to determine if lipid peroxidation is a molecular mechanism important in causing the observed alterations in lipoprotein-cholesterol levels. Human and animal LDL will be subjected to a variety of in vitro peroxidation systems and the plasma clearance and uptake of the peroxidized LDL compared to that of LDL chemically modified by well characterized procedures such as reductive methylation of lysine residues. This work may have health related significance because the damaging process of lipid peroxidation appears to be an important factor in the etiology of human atherosclerosis and cardiomyopathy.

抗氧化营养素在脂质代谢中的作用尚未得到很好的证实 定义的。 我们发现老鼠的饮食缺乏抗氧化剂 营养素（硒和维生素 E）形成低密度水平升高 脂蛋白胆固醇（LDL-cho）。 LDL-cho 升高的发展 自发性高血压 (SHR) 大鼠饲喂缺乏 1% 胆固醇的饮食 与相比，硒 (Se) 和维生素 E 的含量尤其加速 其他大鼠品系。 我们的主要研究目标是表征和 确定负责的代谢和生化机制 饮食引起的大鼠血浆脂蛋白水平的变化 抗氧化营养素缺乏。 无胆固醇饮食，以及 将采用含有 1% 胆固醇的饮食。 待成为的动物 研究对象是近交系 SHR 大鼠和对照正常血压 (WKY) 大鼠。 我们将 研究脂蛋白组成、LDL 分解代谢和 VLDL 合成 抗氧化剂缺乏和充足的大鼠。 血浆清除率和组织 将在完整动物中测定 LDL 的摄取。 LDL 摄取和 VLDL 将在肝脏灌注系统中研究合成。 第二个长期 目标是确定脂质过氧化是否是一种分子机制 对于引起观察到的脂蛋白胆固醇的变化很重要 水平。 人类和动物的低密度脂蛋白将受到各种体外试验 过氧化系统以及过氧化氢的血浆清除和摄取 LDL 与经充分表征的化学修饰 LDL 进行比较 诸如赖氨酸残基的还原甲基化等程序。 这项工作可能 由于脂质的破坏过程具有与健康相关的意义 过氧化似乎是人类病因学的一个重要因素 动脉粥样硬化和心肌病。