MINORITY SCHOOL FACULTY DEVELOPMENT AWARD

抗氧化营养素在脂类代谢中的作用一直不是很好。 已定义。我们发现，喂食缺乏抗氧化剂的饮食的大鼠 营养素(硒和维生素E)会导致低密度水平升高 脂蛋白胆固醇(LDL-CHO)。低密度脂蛋白-胆固醇升高的研究进展 自发性高血压(SHR)大鼠喂饲1%低胆固醇饮食 在硒(Se)和维生素E中，与 其他品系的老鼠。我们的主要研究目标是描述和描述 确定代谢和生化机制(S)负责 饮食对大鼠血浆脂蛋白水平的影响 缺乏抗氧化营养素。无胆固醇饮食，以及 将使用含有1%胆固醇的饮食。未来的动物 研究对象为近交系SHR大鼠和正常血压(WKY)大鼠。我们会 研究脂蛋白组成、低密度脂蛋白分解代谢和极低密度脂蛋白合成 抗氧化剂缺乏和充足的大鼠。血浆清除量和组织 完整的动物对低密度脂蛋白的摄取将被测定。低密度脂蛋白摄取与极低密度脂蛋白 合成将在肝脏灌流系统中进行研究。第二个长期计划 目的是确定脂质过氧化是否是一种分子机制 在引起观察到的脂蛋白胆固醇变化中的重要作用 级别。人和动物的低密度脂蛋白在体外会受到各种 过氧化体系与过氧化产物的血浆清除和摄取 低密度脂蛋白与低密度脂蛋白化学修饰的比较 如赖氨酸残基的还原甲基化等程序。这项工作可能会 具有与健康相关的意义，因为脂质的破坏过程 过氧化似乎是人类病因中的一个重要因素。 动脉粥样硬化和心肌病。

The role of antioxidant nutrients in lipid metabolism has not been well defined. We have found that rats fed a diet deficient in antioxidant nutrients (selenium and vitamin E) develop elevated levels of low density lipoprotein-cholesterol (LDL-cho). The development of elevated LDL-cho in spontaneously hypertensive (SHR) rats fed a 1% cholesterol diet deficient in selenium (Se) and vitamin E is particularly accelerated compared with other rat strains. Our primary research objective is to characterize and determine the metabolic and biochemical mechanism(s) responsible for the alterations in rat plasma lipoprotein levels resulting from dietary deficiencies of antioxidant nutrients. Cholesterol free diets, as well as diets containing 1% cholesterol will be utilized. The animals to be studied are inbred SHR rats, and control normotensive (WKY) rats. We will study lipoprotein composition, LDL catabolism, and VLDL synthesis in antioxidant deficient and sufficient rats. The plasma clearance and tissue uptake of LDL will be determined in intact animals. LDL uptake and VLDL synthesis will be studied in a liver perfusion system. A second long term goal is to determine if lipid peroxidation is a molecular mechanism important in causing the observed alterations in lipoprotein-cholesterol levels. Human and animal LDL will be subjected to a variety of in vitro peroxidation systems and the plasma clearance and uptake of the peroxidized LDL compared to that of LDL chemically modified by well characterized procedures such as reductive methylation of lysine residues. This work may have health related significance because the damaging process of lipid peroxidation appears to be an important factor in the etiology of human atherosclerosis and cardiomyopathy.