MINORITY SCHOOL FACULTY DEVELOPMENT AWARD

少数族裔学校教师发展奖

• 批准号: 3088295
• 负责人: William L Stone
• 金额: $ 5.73万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-08-01 至 1989-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088295
• 关键词: antioxidants; blood lipoprotein biosynthesis; blood lipoprotein metabolism; dietary lipid; dietary trace element; human tissue; hyperlipoproteinemia; laboratory rat; lipid peroxides; liver metabolism; low density lipoprotein; malnutrition; nutrition related tag; peroxidation; selenium; spontaneous hypertensive rat; very low density lipoprotein; vitamin E deficiency

The role of antioxidant nutrients in lipid metabolism has not been well defined. We have found that rats fed a diet deficient in antioxidant nutrients (selenium and vitamin E) develop elevated levels of low density lipoprotein-cholesterol (LDL-cho). The development of elevated LDL-cho in spontaneously hypertensive (SHR) rats fed a 1% cholesterol diet deficient in selenium (Se) and vitamin E is particularly accelerated compared with other rat strains. Our primary research objective is to characterize and determine the metabolic and biochemical mechanism(s) responsible for the alterations in rat plasma lipoprotein levels resulting from dietary deficiencies of antioxidant nutrients. Cholesterol free diets, as well as diets containing 1% cholesterol will be utilized. The animals to be studied are inbred SHR rats, and control normotensive (WKY) rats. We will study lipoprotein composition, LDL catabolism, and VLDL synthesis in antioxidant deficient and sufficient rats. The plasma clearance and tissue uptake of LDL will be determined in intact animals. LDL uptake and VLDL synthesis will be studied in a liver perfusion system. A second long term goal is to determine if lipid peroxidation is a molecular mechanism important in causing the observed alterations in lipoprotein-cholesterol levels. Human and animal LDL will be subjected to a variety of in vitro peroxidation systems and the plasma clearance and uptake of the peroxidized LDL compared to that of LDL chemically modified by well characterized procedures such as reductive methylation of lysine residues. This work may have health related significance because the damaging process of lipid peroxidation appears to be an important factor in the etiology of human atherosclerosis and cardiomyopathy.

抗氧化营养素在脂质代谢中的作用一直不太清楚 定义了 我们已经发现，喂食缺乏抗氧化剂的食物的老鼠 营养素（硒和维生素E）的低密度水平升高 脂蛋白胆固醇（LDL-cho）。 高密度脂蛋白胆固醇的发展， 自发性高血压（SHR）大鼠喂食1%胆固醇缺乏的饮食 硒（Se）和维生素E的摄入量特别快， 其他老鼠品种 我们的主要研究目标是表征和 确定代谢和生化机制（S）负责 饮食引起的大鼠血浆脂蛋白水平的改变 缺乏抗氧化营养素。 无胆固醇饮食，以及 将使用含有1%胆固醇的饮食。 未来的动物 研究的是近交系SHR大鼠和对照正常血压（WKY）大鼠。 我们将 研究脂蛋白组成、LDL催化剂和VLDL合成， 抗氧化剂缺乏和充足的大鼠。 血浆清除率和组织 将在完整动物中测定LDL的摄取。 LDL摄取和VLDL 将在肝灌注系统中研究合成。 的第二长期 目的是确定脂质过氧化是否是一种分子机制 在引起脂蛋白-胆固醇 程度. 人和动物LDL将在体外进行各种实验， 过氧化系统和血浆清除和吸收的过氧化 与通过充分表征的化学修饰的LDL相比， 程序，如赖氨酸残基的还原甲基化。 这项工作可能 具有与健康相关的意义，因为脂质的破坏过程 过氧化反应似乎是人类肝硬化病因学中的一个重要因素 动脉粥样硬化和心肌病。

项目成果

The role of antioxidant nutrients in preventing hyperbaric oxygen damage to the retina.

抗氧化营养素在防止高压氧对视网膜造成损伤方面的作用。

• DOI: 10.1016/0891-5849(89)90043-9
• 发表时间: 1989
• 期刊: Free radical biology & medicine
• 影响因子: 7.4
• 作者: Stone,WL;Henderson,RA;HowardJr,GH;Hollis,AL;Payne,PH;Scott,RL
• 通讯作者: Scott,RL

The effects of in vitro lipid peroxidation on the activity of rat liver microsomal glutathione S-transferase from rats supplemented or deficient in antioxidants.

体外脂质过氧化对补充或缺乏抗氧化剂的大鼠肝微粒体谷胱甘肽S-转移酶活性的影响。

• DOI: 10.1016/0024-3205(88)90079-3
• 发表时间: 1988
• 期刊: Life sciences
• 影响因子: 6.1
• 作者: Harris,CM;Stone,WL
• 通讯作者: Stone,WL

Fatty acid composition of umbilical arteries and veins: possible implications for the fetal EFA-status.

脐动脉和静脉的脂肪酸组成：对胎儿 EFA 状态的可能影响。

• DOI: 10.1007/bf02535131
• 发表时间: 1989
• 期刊: Lipids
• 影响因子: 1.9
• 作者: Hornstra,G;vanHouwelingen,AC;Simonis,M;Gerrard,JM
• 通讯作者: Gerrard,JM