HEMOGLOBIN SYNTHESIS IN THE HUMAN ERYTHROCYTE

人类红细胞中的血红蛋白合成

• 批准号: 3087607
• 负责人: MAHER ALBITAR
• 金额: $ 6.23万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087607
• 关键词: endonuclease; erythrocytes; gel electrophoresis; gene expression; gene interaction; genetic enhancer element; genetic manipulation; genetic terminator element; genetic transcription; globin; globulins; hemoglobin; hemoprotein biosynthesis; human genetic material tag; human tissue; molecular genetics; nucleic acid sequence; radionuclides; tissue /cell culture; transfection

The specific goals of the research component of this proposal are to define the interactions of the functional genes in the human alpha globin gene cluster. Phase I studies will attempt to define and optimize experimental systems in which to study the expression of these genes and in Phase II these studies will be extended to deal with specific mechanisms of gene control including; position effects, transcriptional interference, 3' control elements (transcriptional termination signals and enhancers), and the general impact of one genes transcription upon the function of other genes in the cluster. The results will be related to the mechanisms of globin gene switching and general mechanisms of gene control in tightly spaced clusters. The training which is planned, and the specific experimental system to be explored are designed to interact and provide that applicant with a firm background for future independent work.

本提案研究部分的具体目标是