HEMOGLOBIN SYNTHESIS IN THE HUMAN ERYTHROCYTE

人类红细胞中的血红蛋白合成

• 批准号: 3087604
• 负责人: MAHER ALBITAR
• 金额: $ 6.53万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3087604
• 关键词: endonuclease; erythrocytes; gel electrophoresis; gene expression; gene interaction; genetic enhancer element; genetic manipulation; genetic terminator element; genetic transcription; globin; globulins; hemoglobin; hemoprotein biosynthesis; human tissue; molecular genetics; nucleic acid sequence; radionuclides; tissue /cell culture; transfection

The specific goals of the research component of this proposal are to define the interactions of the functional genes in the human alpha globin gene cluster. Phase I studies will attempt to define and optimize experimental systems in which to study the expression of these genes and in Phase II these studies will be extended to deal with specific mechanisms of gene control including; position effects, transcriptional interference, 3' control elements (transcriptional termination signals and enhancers), and the general impact of one genes transcription upon the function of other genes in the cluster. The results will be related to the mechanisms of globin gene switching and general mechanisms of gene control in tightly spaced clusters. The training which is planned, and the specific experimental system to be explored are designed to interact and provide that applicant with a firm background for future independent work.

本提案研究部分的具体目标是 为了确定人类功能基因之间的相互作用 阿尔法珠蛋白基因簇。第一阶段研究将尝试 定义和优化实验系统，以研究 这些基因的表达，在第二阶段，这些研究将 扩展到处理基因控制的特定机制 包括：位置效应、转录干扰、3‘ 控制元件(转录终止信号和 增强子)，以及一个基因转录的一般影响 依赖于簇中其他基因的功能。结果是 将与珠蛋白基因切换和 紧密排列的簇中基因控制的一般机制。 有计划的培训，以及具体的实验 要探索的系统被设计成交互并提供 申请人对未来的独立工作有扎实的背景。