DETECTION OF CARCINOGEN RESISTANT CELLS IN ORAL MUCOSA

口腔粘膜中抗癌细胞的检测

• 批准号: 3088595
• 负责人: JERRY W HUSSONG
• 金额: $ 5.09万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-01-01 至 1990-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088595
• 关键词: carcinogenesis inhibitor; cheek pouch technique; chemical carcinogen; early diagnosis; hamsters; keratinocyte; neoplasm /cancer diagnosis; oral mucosa; oral pharyngeal neoplasm; preneoplastic state

The specific aims of the proposed research are: 1) to identify chemical agents which acutely inhibit replication of hamster buccal pouch keratinocytes (HBPE cells) in vitro, and do not induce resistant cell variants during the period of acute chemical exposure, and 2) to use the chemical agents identified in Specific Aim 1 to select in vitro, and thereby identify and quantitate colonies of HBPE cells which have undergone one or more steps in the carcinogenic process. The proposed assay for initiated HBPE cell is based on the hypothesis that during initiation in vivo, cells acquire a relative resistance to the cytotoxic effects of the inducing carcinogen. The long-range goal of the proposed research is to develop improved strategies for the biological and biochemical characterization of precancerous cells in oral mucosa, and for the early detection and prevention of human oral cancer.

拟议研究的具体目标是：1）确定化学物质 急性抑制仓鼠颊囊复制的药物 体外角质形成细胞（HBPE细胞），并且不诱导耐药细胞 急性化学暴露期间的变异，以及2）使用 具体目标 1 中确定的化学试剂用于体外选择，以及 从而鉴定和定量经历了 HBPE 细胞集落 致癌过程中的一个或多个步骤。 拟议的测定 启动的 HBPE 细胞基于这样的假设：在启动过程中 在体内，细胞获得对细胞毒性作用的相对抵抗力 诱发致癌物质。 拟议研究的长期目标是 制定生物和生化的改进策略 口腔粘膜癌前细胞的特征，以及早期发现 人类口腔癌的检测和预防。